Doktorsavhandlingar / Doctoral Theses - H2 (BioNut): Recent submissions
Now showing items 81-100 of 299
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Analysis of DNA adducts formed by tamoxifen and related antiestrogenic and estrogenic compounds in rats
(Biovetenskaper och näringslära / Biosciences and Nutrition, 2000-05-19)The work presented herein has focused on DNA adduct formation by the non-steroidal antiestrogen tamoxifen and its analogues. Tamoxifen, [(Z)-1-{4-[2-(dimethylamino)-ethoxy]phenyl}-1,2-diphenyl-1-butene], is widely used in ... -
HPRT mutational spectra and microsatellite DNA instability in HNPCC and lung cancer patients
(Biovetenskaper och näringslära / Biosciences and Nutrition, 2000-05-25)The, general aims of this work have been to explore the use of microsatellite DNA length variation and mutational spectra of the hypoxanthine guanine phosphoribosyltransferase (HPRT) locus in T-cells as tools for a better ... -
Signal transduction via estrogen receptors (ERs) and estrogen receptor-related receptors (ERRs)
(Institutionen för medicinsk näringslära / Department of Medical Nutrition, 2000-05-02)Estrogens influence post-natal development and function of the female reproductive system and other organs including bone and cardiovascular system. Estrogens are also involved in the development and growth of certain ... -
Functional analysis of ligand recognition by the glucocorticoid receptor
(Institutionen för medicinsk näringslära / Department of Medical Nutrition, 2000-04-14)The glucocorticoid receptor (GR) is a ligand-inducible transcription factor that belongs to the superfamily of nuclear hormone receptors. It contains three structural and functional domains. The C-terminal domain binds the ... -
Expression and function of ribozymes in transgenic mice
(Institutionen för medicinsk näringslära / Department of Medical Nutrition, 2000-04-06)Hammerhead and hairpin ribozymes are short RNAs that act as sequence-specific endoribonucleases by cleaving a target RNA after hybridization to complementary sequences. By changing the specific target hybridization sequence ... -
Towards ribozyme-mediated gene therapy of HIV-1 infections
(Institutionen för medicinsk näringslära / Department of Medical Nutrition, 2000-02-04)HIV-1 is the causative agent in AIDS, the world's fourth most frequent cause of death. HIV is a retrovirus that primarily infects macrophages and T helper cells - required for a proper immune response. An infection is ... -
Chromatin remodeling complexes involved in gene activation by the glucocorticoid receptor
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-11-26)The glucocorticoid receptor (GR) belongs to a large family of ligand-inducible nuclear receptors and consists of a ligand binding domain, a DNA binding domain and transactivation domains. The main transcriptional activation ... -
P-type ATPases studied by electron microscopy and two-dimensional crystallization
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-11-24)Electron microscopy and two-dimensional crystallography have been used to study the molecular structures of the porcine renal Na+,K+-ATPase and the porcine gastric H+,K+-ATPase. In addition, morphological changes have been ... -
Conformational properties of transmembrane polypeptide segments in the ER membrane
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-11-12)We have developed a new experimental approach where the active site of oligosaccharyl transferase is used as a point of reference against which the position of a transmembrane segment in the membrane can be measured. This ... -
Quantitative analysis of DNA adducts formed by some epoxides of industrial importance
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-10-22)The work presented herein has focused on DNA adduct formation by three industrial epoxides, allyl glycidyl ether, propylene oxide and epichlorohydrin. Epoxides, owing to the strained oxirane ring are chemically reactive ... -
Electron crystallography of soluble proteins
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-05-07)Protein structures at moderate to high resolution can now be readily solved using an electron microscope and image processing techniques. The averaging conditions required to overcome the low signal to noise ratio of ... -
Structural analysis of microsomal glutathione transferase 1 by electron crystallography
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-04-21)The goals of this project were the two-dimensional crystallization of the membrane protein microsomal glutathione transferase 1 and the three-dimensional structure determination by electron crystallography. Microsomal ... -
X-ray structure analysis of short-chain dehydrogenases/reductases
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-04-26)X-ray crystallography and site-directed mutagenesis were used to better understand the structure/function relationships in the family of short-chain dehydrogenases/reductases (SDR). This group of enzymes constitutes a ... -
Crystallographic studies on redox enzymes containing the thioredoxin fold
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1999-01-15)Three-dimensional structures of two redox enzymes, glutathione peroxidase from human plasma and a protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus, have been determined by X-ray crystallography. ... -
UV-induced DNA damage in humans
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1998-12-25)Ultraviolet radiation is considered to be the most harmful part of solar energy affecting man. The depletion of the ozone layer around the Earth increases the total exposure to UV-light. The incidence of skin cancer in man ... -
Transcription factors involved in negative and positive gene regulation by glucocorticoids
(Institutionen för medicinsk näringslära / Department of Medical Nutrition, 1998-12-25)Steroid hormones, including glucocorticoids (GCs), control a wide variety of developmental and physiological responses in higher eukaryotic organisms. They pass through the plasma membrane and bind to specific receptor ... -
Semliki forest virus-derived packaging system for production of retroviral vectors
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1998-11-20)Gene therapy is a treatment of disease by transfer of genetic material into somatic cells of an individual. Successful gene therapy depends on a delivery system which can efficiently transfer the gene into target cells and ... -
32P-postlabelling analysis of complex DNA adducts in human tissues
(Biovetenskaper och näringslära / Biosciences and Nutrition, 1998-11-20)Complex DNA adducts in humans originate from exogenous and endogenous sources. Polycyclic aromatic hydrocarbons (PAHs) are a large group of chemicals including many potent carcinogens. PAHs are ubiquitously present in the ... -
The use of molecular techniques for identification of genetic divergence in transplantation : with special reference to MHC genes and HLA typing
(Inst för biovetenskaper och näringslära / Dept of Biosciences and Nutrition, 1998-11-06)Transplantation immunology basically deals with the immune mechanisms that lead to rejection of transplanted tissues. Lymphocytes are the key mediators of graft rejection. At the time for this observation, the genetic loci ... -
Transcription factors regulating the Btk promoter
(Inst för biovetenskaper / Dept of Biosciences, 1997-11-21)Bruton's agammaglobulinemia tyrosine kinase (Btk) is a cytoplasmic tyrosine kinase-related to the Src family of kinases. Mutations in various parts of the gene have been shown to lead to X-linked agammaglobulinemia, an ...