Immune responses related to protection against rotavirus after natural infection and vaccination
Author: Johansen, Kari
Date: 1999-12-10
Location: Skandiasalen, Astrid Lindgrens barnsjukhus, Karolinska sjukhuset
Time: 9.00
Department: Institutionen för kvinnors och barns hälsa / Department of Women's and Children's Health
Abstract
Rotavirus is the most important cause of severe gastroenteritis in
children under five years of age and is estimated to be responsible for
one-third of all diarrhea-associated hospitalizations and 600,000 deaths/
year worldwide. 1500-1700 rotavirus-associated hospitalizations of
children are estimated to occur annually in Sweden (3.7 hospitalization/
1000 children/y). An effective rotavirus vaccine early in life would have
a beneficial effect on child health. Oral vaccine candidates have
provided good (63-89%) but not complete protection. The mechanisms and
proteins involved in mediating protective immunity are still unresolved.
The role of immunoglobulin A (IgA) in relation to protective immunity was
evaluated. A method for purification of human IgA, utilizing the
galactose-binding lectin jacalin from the jack-fruit Artrocarpus
integrifolia, was used for determination of IgA antibody specificity for
different rotavirus proteins and the role of IgA in the neutralization of
rotavirus. In convalescent sera from children with an acute rotavirus
infection, serum IgA antibodies were detected against VP2, VP4, VP6, VP7,
NSP2, and/or NSP3. Serum IgA antibodies purified from rotavirus
seropositive individuals were shown in vitro to neutralize unattached and
attached rotavirus. A library of monoclonal secretory IgA antibodies
directed against the neutralizing antigen, VP4 of rotavirus, were shown
to protect mice against rotavirus induced diarrhea and neutralize
rotavirus after transcytosis through epithelial cells.
Rotavirus non-structural protein, NSP4, was recently suggested to
function as a viral enterotoxin and to play a role in the induction of
diarrhea. The ability of rotavirus NSP4 to induce a humoral and
cell-mediated immune response was examined. NSP4 stimulated a
cell-mediated immune response in naturally exposed adults, and a modest
humoral immune response in naturally infected children, vaccinated
children (oral RRV-TV), and naturally exposed adults.
Since the oral rotavirus vaccine candidates provide good but not complete
protection; the possibility of parenteral immunization with formalin
inactivated rotavirus (I-RRV) was evaluated. I-RRV was administered three
times intramuscularly to mice and induced a good IgG response,
neutralizing antibodies and protective immunity. The two adjuvants tested
with a similar immunization schedule, MPL® and monooleate/lauric acid,
induced higher IgG antibodies, slightly higher neutralizing antibody
titers and protective immunity. The last adjuvant tested, PCPP, induced
~10-fold higher IgG antibody titers and neutralizing antibody titers as
well as induced protective immunity after two doses in a dose-dependent
manner compared to inactivated virus alone.
List of papers:
I. Johansen K, Granqvist L, Karlen K, Stintzing G, Uhnoo I, Svensson L (1994). "Serum IgA immune response to individual rotavirus polypeptides in young children with rotavirus infection." Arch Virol 138(3-4): 247-259
Pubmed
II. Johansen K, Svensson L (1997). "Neutralization of rotavirus and recognition of immunologically important epitopes on VP4 and VP7 by human IgA" Arch Virol 142(7): 1491-1498
Pubmed
III. Ruggeri FM, Johansen K, Basile G, Kraehenbuhl JP, Svensson L (1998). "Antirotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice from diarrhea" J Virol 72(4): 2708-2714
Pubmed
IV. Johansen K, Hinkula J, Espinoza F, Levi M, Zeng C, Ruden U, Vesikari T, Estes M, Svensson L Department of Women and Child Health, Karolinska Hospital, Karolinska (1999). "Humoral and cell-mediated immune responses in humans to the NSP4 enterotoxin of rotavirus. " J Med Virol 59(3): 369-377
Pubmed
V. Johansen K, Schroder U, Svensson L (1970). "Immunogenicity and protective efficacy of a formalin-inactivated rotavirus vaccine with and without lipid adjuvants" (Manuscript)
VI. Johansen K, Jenkins S, Payne L, Ramstedt U, Schroder U, Svensson L (1970). "Polyphosphazene (PCPP)is a potent adjuvant for a formalin-inactivated rotavirus" (Manuscript)
I. Johansen K, Granqvist L, Karlen K, Stintzing G, Uhnoo I, Svensson L (1994). "Serum IgA immune response to individual rotavirus polypeptides in young children with rotavirus infection." Arch Virol 138(3-4): 247-259
Pubmed
II. Johansen K, Svensson L (1997). "Neutralization of rotavirus and recognition of immunologically important epitopes on VP4 and VP7 by human IgA" Arch Virol 142(7): 1491-1498
Pubmed
III. Ruggeri FM, Johansen K, Basile G, Kraehenbuhl JP, Svensson L (1998). "Antirotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice from diarrhea" J Virol 72(4): 2708-2714
Pubmed
IV. Johansen K, Hinkula J, Espinoza F, Levi M, Zeng C, Ruden U, Vesikari T, Estes M, Svensson L Department of Women and Child Health, Karolinska Hospital, Karolinska (1999). "Humoral and cell-mediated immune responses in humans to the NSP4 enterotoxin of rotavirus. " J Med Virol 59(3): 369-377
Pubmed
V. Johansen K, Schroder U, Svensson L (1970). "Immunogenicity and protective efficacy of a formalin-inactivated rotavirus vaccine with and without lipid adjuvants" (Manuscript)
VI. Johansen K, Jenkins S, Payne L, Ramstedt U, Schroder U, Svensson L (1970). "Polyphosphazene (PCPP)is a potent adjuvant for a formalin-inactivated rotavirus" (Manuscript)
Issue date: 1999-11-19
Publication year: 1999
ISBN: 91-628-3882-2
Statistics
Total Visits
Views | |
---|---|
Immune ...(legacy) | 522 |
Immune ... | 69 |
Total Visits Per Month
July 2020 | August 2020 | September 2020 | October 2020 | November 2020 | December 2020 | January 2021 | |
---|---|---|---|---|---|---|---|
Immune ... | 8 | 1 | 2 | 7 | 1 | 5 | 2 |
Top country views
Views | |
---|---|
United States | 105 |
China | 87 |
Germany | 43 |
Sweden | 20 |
India | 10 |
Japan | 10 |
South Korea | 8 |
Belgium | 7 |
Indonesia | 7 |
Russia | 6 |
Top cities views
Views | |
---|---|
Sunnyvale | 29 |
Beijing | 19 |
Kiez | 18 |
Shenzhen | 11 |
Woodbridge | 9 |
Seoul | 7 |
Jakarta | 5 |
Houston | 4 |
Mumbai | 4 |
Bruges | 3 |