Linking transcription to DNA replication in mammalian mitochondria
Author: Shi, Yonghong
Date: 2013-02-22
Location: Konferensrum Tellus, Plan 5 av Reziuslaboratoriet, Scheeles väg 1, KI Solna
Time: 13:00
Department: Inst för laboratoriemedicin / Dept of Laboratory Medicine
Abstract
Most of the adenosine phosphate (ATP) used to drive cellular processes is produced through oxidative phosphorylation (OXPHOS). At the heart of this process is the electron transport chain, which contains a number of protein complexes located in the inner membrane of mitochondria. In addition to about 90 nuclear encoded gene products, the electron transport chain also contains 13 subunits encoded by mitochondrial DNA (mtDNA). This double-stranded, circular DNA genome is therefore essential for proper OXPHOS function; mutations, deletion, or depletion of mtDNA can cause mitochondrial dysfunction and human disease.
The mtDNA molecule is replicated and transcribed by molecular machineries that are distinct from those present in the nucleus. Many of the components are related to proteins identified in bacteria and bacteriophages, in keeping with the prokaryotic origin of the mitochondrion. The transcription machinery in mammalian mitochondria contains three essential factors: mitochondrial RNA polymerase (POLRMT), mitochondrial transcription factor A (TFAM), and mitochondrial transcription factor B (TFB2M). Recently, the role of TFAM as a core initiation factor has been questioned. In this thesis, we demonstrate that both TFAM and TFB2M are required for mitochondrial transcription. We also demonstrate that TFB1M, a TFB2M paralog, does not function as a bona fide transcription factor. Even if TFAM is an essential factor for transcription initiation in vitro, the absolute requirement of TFAM can be circumvented by experimental conditions that stimulate DNA breathing at the promoter. We suggest a possible explanation for this effect, by demonstrating that TFAM can introduce negative supercoils and cause conformational changes in mtDNA that facilitates promoter recognition and transcription initiation by POLRMT and TFB2M.
Our studies also demonstrate that a substantial fraction of the transcription events initiated from the light strand promoter (LSP) are prematurely terminated at a conserved sequence element, CSBII. The 3 -ends of these transcripts correspond to prominent RNA-DNA transition sites mapped in vivo, strongly suggesting that they are used to prime mitochondrial DNA synthesis.
The mitochondrial transcription termination factor (MTERF1) was originally identified as a regulator of rRNA transcription. MTERF1 binds to a 28-bp mtDNA sequence downstream of the 16S rRNA gene. MTERF1 supposedly terminates transcription at this location to produce more rRNAs than the mRNAs species encoded by down stream sequences on the same strand. In this thesis, we explore an alternative role for MTERF1 as a regulator of mtDNA replication. We demonstrate that binding of MTERF1 causes a pause in the progression of the mitochondrial replication fork in one direction, but not in the other. The effect is explained by MTERF1’s ability to counteract the DNA unwinding activity of the mitochondrial DNA helicase, TWINKLE. Based on our findings, we speculate that MTERF1 can coordinate synthesis of the two mtDNA strands.
The mtDNA molecule is replicated and transcribed by molecular machineries that are distinct from those present in the nucleus. Many of the components are related to proteins identified in bacteria and bacteriophages, in keeping with the prokaryotic origin of the mitochondrion. The transcription machinery in mammalian mitochondria contains three essential factors: mitochondrial RNA polymerase (POLRMT), mitochondrial transcription factor A (TFAM), and mitochondrial transcription factor B (TFB2M). Recently, the role of TFAM as a core initiation factor has been questioned. In this thesis, we demonstrate that both TFAM and TFB2M are required for mitochondrial transcription. We also demonstrate that TFB1M, a TFB2M paralog, does not function as a bona fide transcription factor. Even if TFAM is an essential factor for transcription initiation in vitro, the absolute requirement of TFAM can be circumvented by experimental conditions that stimulate DNA breathing at the promoter. We suggest a possible explanation for this effect, by demonstrating that TFAM can introduce negative supercoils and cause conformational changes in mtDNA that facilitates promoter recognition and transcription initiation by POLRMT and TFB2M.
Our studies also demonstrate that a substantial fraction of the transcription events initiated from the light strand promoter (LSP) are prematurely terminated at a conserved sequence element, CSBII. The 3 -ends of these transcripts correspond to prominent RNA-DNA transition sites mapped in vivo, strongly suggesting that they are used to prime mitochondrial DNA synthesis.
The mitochondrial transcription termination factor (MTERF1) was originally identified as a regulator of rRNA transcription. MTERF1 binds to a 28-bp mtDNA sequence downstream of the 16S rRNA gene. MTERF1 supposedly terminates transcription at this location to produce more rRNAs than the mRNAs species encoded by down stream sequences on the same strand. In this thesis, we explore an alternative role for MTERF1 as a regulator of mtDNA replication. We demonstrate that binding of MTERF1 causes a pause in the progression of the mitochondrial replication fork in one direction, but not in the other. The effect is explained by MTERF1’s ability to counteract the DNA unwinding activity of the mitochondrial DNA helicase, TWINKLE. Based on our findings, we speculate that MTERF1 can coordinate synthesis of the two mtDNA strands.
List of papers:
I. Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. Litonin D, Sologub M, Shi Y, Savkina M, Anikin M, Falkenberg M, Gustafsson CM, Temiakov D. J Biol Chem. 2010 Jun 11;285(24):18129-33.
Fulltext (DOI)
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II. Mammalian transcription factor A is a core component of the mitochondrial transcription machinery. Shi Y, Dierckx A, Wanrooij PH, Wanrooij S, Larsson NG, Wilhelmsson LM, Falkenberg M, Gustafsson CM. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16510-5.
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III. Conserved sequence box II directs transcription termination and primer formation in mitochondria.Pham XH, Farge G, Shi Y, Gaspari M, Gustafsson CM, Falkenberg M. J Biol Chem. 2006 Aug 25;281(34):24647-52.
Fulltext (DOI)
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IV. MTERF1 is a contra-helicase that stimulates DNA replication fork pausing in vitro and in vivo. Shi Y, Zhu X, Falkenberg M, Gustafsson CM. [Manuscript]
I. Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. Litonin D, Sologub M, Shi Y, Savkina M, Anikin M, Falkenberg M, Gustafsson CM, Temiakov D. J Biol Chem. 2010 Jun 11;285(24):18129-33.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Mammalian transcription factor A is a core component of the mitochondrial transcription machinery. Shi Y, Dierckx A, Wanrooij PH, Wanrooij S, Larsson NG, Wilhelmsson LM, Falkenberg M, Gustafsson CM. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16510-5.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Conserved sequence box II directs transcription termination and primer formation in mitochondria.Pham XH, Farge G, Shi Y, Gaspari M, Gustafsson CM, Falkenberg M. J Biol Chem. 2006 Aug 25;281(34):24647-52.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. MTERF1 is a contra-helicase that stimulates DNA replication fork pausing in vitro and in vivo. Shi Y, Zhu X, Falkenberg M, Gustafsson CM. [Manuscript]
Institution: Karolinska Institutet
Supervisor: Gustafsson, Claes Jr
Issue date: 2013-02-01
Rights:
Publication year: 2013
ISBN: 978-91-7549-063-2
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