Genetic regulation of neuroinflammation after infection and injury
Author: Abdelmagid, Nada Omar
Date: 2012-06-08
Location: Föreläsningssalen CMM, L8:00, Karolinska Universitetssjukhuset, Solna
Time: 09.00
Department: Inst för klinisk neurovetenskap / Dept of Clinical Neuroscience
Abstract
Neuroinflammation is a common theme in a spectrum of central nervous system (CNS) diseases resulting in neuronal damage and degeneration. The exact mechanisms regulating inflammation in the CNS or its consequences in terms of nerve injury are still not known in detail. However, it is known that many inflammatory conditions at least in part are regulated by genetic factors. Exact definition of these factors will provide a better understanding of underlying mechanisms. The focus of this thesis has been to explore the genetic regulation of neuroinflammation in viral infections and mechanical nerve injuries using experimental models.
The genetic regulation of susceptibility to Herpes simplex type-1 encephalitis (HSE), a devastating condition that affects humans, was investigated in different inbred rat strains. Using a series of different experimental approaches we succeeded in defining two different candidate genes that regulate susceptibility to HSE. Interestingly, these genetic influences act at two different stages of neurotropic HSV-1 virus CNS entry. Thus, the calcitonin receptor (Calcr) gene was identified as a candidate for peripheral neuronal infection and propagation to the CNS, while the von Willebrand factor (Vwf) gene was identified as a candidate for disease progression in the CNS and blood-brain barrier (BBB) dysfunction. The latter has previously been associated to cerebral malaria infection by Plasmodium falciparum and endothelial cell activation, suggesting that this gene is important in several human infectious conditions. More detailed histopathological and molecular studies of HSV-1 propagation, immune cell recruitment and inflammatory changes was the focus of another study, which provides further support for the notion that entry of virus into the perineurium is an important step regulating susceptibility for virus propagation into the CNS. Also, the differences found between the studied strains in relation to immune activation and responses in the peripheral nervous system (PNS) and CNS clearly demonstrate that genetic factors regulate virus - host interactions. Thus, our studies on HSE have provided several new perspectives of how susceptibility to HSV-1 virus can be regulated at different levels, as well as identifying two different candidate genes, all of which can serve as basis for further studies of human HSE.
In a fourth study, the genetic regulation of the response to a mechanical nerve injury was explored in order to identify regulatory pathways for innate immune responses occurring without an infectious trigger. We identified two quantitative trait loci (QTLs) on chromosome 1 (Neuinflam4) and 7 (Neuinflam5), respectively, regulating major histocompatibility complex class II (MHC II) expression after ventral root avulsion. Additionally, another QTL (Neuinflam9) on chromosome 10 regulated the expression of several important innate immune genes, including C1q, Il1β, Tlr2 and Irf7. The chromosome 10 region containing Neuinflam9 overlaps with Toxo1, which regulates resistance to Toxoplasma gondii infection. Interestingly, a part of this QTL conferred resistance to experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS) indicating that this QTL could regulate susceptibility both to infectious and autoimmune conditions.
In conclusion, the identification of several genetically regulated pathways presented herein provides a basis for further molecular exploration of different conditions characterized by neuroinflammation. This will be a necessary prerequisite for the formulation of new targeted therapeutic interventions that can prevent permanent damage to the nervous system.
The genetic regulation of susceptibility to Herpes simplex type-1 encephalitis (HSE), a devastating condition that affects humans, was investigated in different inbred rat strains. Using a series of different experimental approaches we succeeded in defining two different candidate genes that regulate susceptibility to HSE. Interestingly, these genetic influences act at two different stages of neurotropic HSV-1 virus CNS entry. Thus, the calcitonin receptor (Calcr) gene was identified as a candidate for peripheral neuronal infection and propagation to the CNS, while the von Willebrand factor (Vwf) gene was identified as a candidate for disease progression in the CNS and blood-brain barrier (BBB) dysfunction. The latter has previously been associated to cerebral malaria infection by Plasmodium falciparum and endothelial cell activation, suggesting that this gene is important in several human infectious conditions. More detailed histopathological and molecular studies of HSV-1 propagation, immune cell recruitment and inflammatory changes was the focus of another study, which provides further support for the notion that entry of virus into the perineurium is an important step regulating susceptibility for virus propagation into the CNS. Also, the differences found between the studied strains in relation to immune activation and responses in the peripheral nervous system (PNS) and CNS clearly demonstrate that genetic factors regulate virus - host interactions. Thus, our studies on HSE have provided several new perspectives of how susceptibility to HSV-1 virus can be regulated at different levels, as well as identifying two different candidate genes, all of which can serve as basis for further studies of human HSE.
In a fourth study, the genetic regulation of the response to a mechanical nerve injury was explored in order to identify regulatory pathways for innate immune responses occurring without an infectious trigger. We identified two quantitative trait loci (QTLs) on chromosome 1 (Neuinflam4) and 7 (Neuinflam5), respectively, regulating major histocompatibility complex class II (MHC II) expression after ventral root avulsion. Additionally, another QTL (Neuinflam9) on chromosome 10 regulated the expression of several important innate immune genes, including C1q, Il1β, Tlr2 and Irf7. The chromosome 10 region containing Neuinflam9 overlaps with Toxo1, which regulates resistance to Toxoplasma gondii infection. Interestingly, a part of this QTL conferred resistance to experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS) indicating that this QTL could regulate susceptibility both to infectious and autoimmune conditions.
In conclusion, the identification of several genetically regulated pathways presented herein provides a basis for further molecular exploration of different conditions characterized by neuroinflammation. This will be a necessary prerequisite for the formulation of new targeted therapeutic interventions that can prevent permanent damage to the nervous system.
List of papers:
I. The Calcitonin receptor gene is a candidate for regulation of susceptibility to Herpes simplex type 1 neuronal infection leading to encephalitis in rat. Nada Abdelmagid, Biborka Bereczky-Veress, André Ortlieb Guerreiro-Cacais, Petra Bergman, Katarina Luhr, Tomas Bergström, Birgit Sköldenberg, Fredrik Piehl, Tomas Olsson, Margarita Diez. [Accepted]
Pubmed
View record in Web of Science®
II. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. Biborka Bereczky-Veress, Nada Abdelmagid, Fredrik Piehl, Tomas Bergström, Tomas Olsson, Birgit Sköldenberg, Margarita Diez. PLoS One. 2010 Aug 27;5(8).
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Genetic variant of Vwf identified as a candidate gene regulating CNS immune activation and outcome in experimental Herpes simplex encephalitis. Nada Abdelmagid, Biborka Bereczky-Veress, Santosh Atanur, Alena Musilová, Václav Zídek, Mohsen Khademi, Laura Saba, Cécile Denis, Ana Garcia-Diaz, Tomas Bergström, Birgit Sköldenberg, Timothy Aitman, Norbert Hübner, Tomas Olsson, Michal Pravenec, Margarita Diez. [Manuscript]
IV. Identification of gene regions regulating inflammatory microglial response in the rat CNS after nerve injury. Margarita Diez, Nada Abdelmagid, Karin Harnesk, Mikael Ström, Olle Lidman, Maria Swanberg, Rickard Lindblom, Faiez Al-Nimer, Maja Jagodic, Tomas Olsson, Fredrik Piehl. Journal of Neuroimmunology. 2009 Jul 25; 212(1-2):82-92.
Fulltext (DOI)
Pubmed
View record in Web of Science®
I. The Calcitonin receptor gene is a candidate for regulation of susceptibility to Herpes simplex type 1 neuronal infection leading to encephalitis in rat. Nada Abdelmagid, Biborka Bereczky-Veress, André Ortlieb Guerreiro-Cacais, Petra Bergman, Katarina Luhr, Tomas Bergström, Birgit Sköldenberg, Fredrik Piehl, Tomas Olsson, Margarita Diez. [Accepted]
Pubmed
View record in Web of Science®
II. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. Biborka Bereczky-Veress, Nada Abdelmagid, Fredrik Piehl, Tomas Bergström, Tomas Olsson, Birgit Sköldenberg, Margarita Diez. PLoS One. 2010 Aug 27;5(8).
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Genetic variant of Vwf identified as a candidate gene regulating CNS immune activation and outcome in experimental Herpes simplex encephalitis. Nada Abdelmagid, Biborka Bereczky-Veress, Santosh Atanur, Alena Musilová, Václav Zídek, Mohsen Khademi, Laura Saba, Cécile Denis, Ana Garcia-Diaz, Tomas Bergström, Birgit Sköldenberg, Timothy Aitman, Norbert Hübner, Tomas Olsson, Michal Pravenec, Margarita Diez. [Manuscript]
IV. Identification of gene regions regulating inflammatory microglial response in the rat CNS after nerve injury. Margarita Diez, Nada Abdelmagid, Karin Harnesk, Mikael Ström, Olle Lidman, Maria Swanberg, Rickard Lindblom, Faiez Al-Nimer, Maja Jagodic, Tomas Olsson, Fredrik Piehl. Journal of Neuroimmunology. 2009 Jul 25; 212(1-2):82-92.
Fulltext (DOI)
Pubmed
View record in Web of Science®
Institution: Karolinska Institutet
Supervisor: Diez, Margarita
Issue date: 2012-05-16
Rights:
Publication year: 2012
ISBN: 978-91-7457-763-1
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