Bronchoalveolar lavage and serum protein patterns in healthy individuals and sarcoidosis patients : a proteomics approach
Author: Sabounchi Schütt, Fariba
Date: 2004-02-13
Location: Thorax Aulan N2:U1, Karolinska Sjukhuset
Time: 9.00
Department: Institutionen för medicin / Department of Medicine
Abstract
The etiology of sarcoidosis remains unknown. Although biochemical changes
are frequently observed in sarcoidosis, the problem of finding distinct
biomarkers remains. Only a few markers are defined in this disease. In
previous studies, proteomics investigations of sarcoidosis have generated
valuable information relevant to an altered protein expression in this
disease. In this thesis the focus has been on soluble components of cell
free bronchoalveolar lavage fluid (BALF) and also serum from patients
with active sarcoidosis as well as healthy individuals. Proteomics has
been applied for the analysis of the samples, for defining and mapping
the specific BALF proteins and to investigate the pattern changes in
these samples in the disease. An approach of comparative proteomics has
been presented. We developed an alternative sample application method
capable of high loading of samples, for the separation of proteins in the
first dimension of two dimensional gel electrophoresis. We optimized a
sample preparation and handling method based on a proteomics approach to
identify specific proteins in the BALF. Normal BALF protein samples were
investigated and the protein contents were mapped in the pH interval
4.5-5.2. Furthermore we analyzed the protein expression changes in BALF
from sarcoidosis patients and compared it to the healthy controls. Twenty
two proteins were found to be altered. Among them many were new for this
sample. Serum proteins of the same patients were also investigated which
resulted in the detection of 21 altered proteins in the patients. In a
search for common protein alternations between the two compartments, we
were able to show a similar expression pattern of three proteins, common
in both serum and BALF.
In conclusion, the proteomics approach used here was successful at defining specific BALF proteins and identifying several new proteins in BALF and serum. Moreover, the establishment of the protein expression pattern in BALF and serum of sarcoidosis may yield useful information in the search for diagnostic and predictive tools in this disease. Finally, the proteomics approach presented here may be applicable also in other pulmonary disorders.
In conclusion, the proteomics approach used here was successful at defining specific BALF proteins and identifying several new proteins in BALF and serum. Moreover, the establishment of the protein expression pattern in BALF and serum of sarcoidosis may yield useful information in the search for diagnostic and predictive tools in this disease. Finally, the proteomics approach presented here may be applicable also in other pulmonary disorders.
List of papers:
I. Sabounchi-Schutt F, Astrom J, Olsson I, Eklund A, Grunewald J, Bjellqvist B (2000). "An immobiline DryStrip application method enabling high-capacity two-dimensional gel electrophoresis. " Electrophoresis 21(17): 3649-56
Pubmed
II. Sabounchi-Schutt F, Astrom J, Eklund A, Grunewald J, Bjellqvist B (2001). "Detection and identification of human bronchoalveolar lavage proteins using narrow-range immobilized pH gradient DryStrip and the paper bridge sample application method. " Electrophoresis 22(9): 1851-60
Pubmed
III. Sabounchi-Schutt F, Astrom J, Hellman U, Eklund A, Grunewald J (2003). "Changes in bronchoalveolar lavage fluid proteins in sarcoidosis: a proteomics approach. " Eur Respir J 21(3): 414-20
Pubmed
IV. Sabounchi Schutt F, Mikko M, Eklund A, Grunewald J, Astrom J (2004). "Serum proteins pattern in sarcoidosis analyzed by a proteomics approach." (Submitted)
View record in Web of Science®
I. Sabounchi-Schutt F, Astrom J, Olsson I, Eklund A, Grunewald J, Bjellqvist B (2000). "An immobiline DryStrip application method enabling high-capacity two-dimensional gel electrophoresis. " Electrophoresis 21(17): 3649-56
Pubmed
II. Sabounchi-Schutt F, Astrom J, Eklund A, Grunewald J, Bjellqvist B (2001). "Detection and identification of human bronchoalveolar lavage proteins using narrow-range immobilized pH gradient DryStrip and the paper bridge sample application method. " Electrophoresis 22(9): 1851-60
Pubmed
III. Sabounchi-Schutt F, Astrom J, Hellman U, Eklund A, Grunewald J (2003). "Changes in bronchoalveolar lavage fluid proteins in sarcoidosis: a proteomics approach. " Eur Respir J 21(3): 414-20
Pubmed
IV. Sabounchi Schutt F, Mikko M, Eklund A, Grunewald J, Astrom J (2004). "Serum proteins pattern in sarcoidosis analyzed by a proteomics approach." (Submitted)
View record in Web of Science®
Issue date: 2004-01-23
Publication year: 2004
ISBN: 91-7349-790-8
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