Pituitary autoantibodies in endocrine disorders
Author: Bensing, Sophie
Date: 2005-10-21
Location: Skandiasalen, Astrid Lindgrens Barnsjukhus, Solna
Time: 9.00
Department: Institutionen för molekylär medicin / Department of Molecular Medicine
View/ Open:
Thesis (258.4Kb)
Abstract
Autoimmune endocrine disorders are characterised by the development of autoantibodies to specific autoantigens in the target organs. Lymphocytic hypophysitis (LyH) is a disease characterised by inflammation of the pituitary gland, often resulting in hypopituitarism. The aetiology of LyH is considered to be autoimmune. However, only a few pituitary autoantigens have so far been identified. Reliable autoantibody markers are requested in the diagnostic procedure of LyH to avoid unnecessary surgical intervention.
The aim of this study was to evaluate the occurrence of pituitary autoantibodies in patients with nonadenomatous pituitary disease and also to identify novel pituitary autoantigens. Autoantibodies to human pituitary cytosolic proteins were determined by immunoblotting. Reactivity to a M, 49 000 pituitary autoantigen was significantly more frequent in patients with idiopathic pituitary hormone deficiency (6/21 (28%) p< 0.05) as well as their relatives (10/35 (28%) p<0.02) compared to control subjects (3/44 (6.8%)). The importance of these pituitary autoantibodies as markers for LyH remains to be confirmed.
Autoantibodies against human pituitary cytosol and ten additional organspecific autoantigens were measured in sera from 30 patients with empty sella syndrome (ESS). None of the autoantibodies tested was more frequently found in ESS patients compared to healthy controls. Thus, by analysing autoantibodies we did not find evidence of ESS being associated with any autoimmune disease.
Autoantibodies to a novel 36-kDa pituitary cytosolic autoantigen were more common in patients with ACTH-deficiency (12/65 (18.5%) compared to control subjects (2/57 3.5%) (p<0.0214). In addition, autoantibodies to thyroglobulin (M) were positively correlated to immunoreactivity against the 36-kDa pituitary autoantigen.
A human pituitary cDNA expression library was successfully constructed. Immunoscreening identified secretogranin II as a pituitary autoantigen in a patient with partial pituitary insufficiency and empty sella.
By immunohistochemistry, autoantibodies against ACTH and gonadotrophs were detected in sera from patients with autoimmune polyendocrine syndrome type 1 (APS1) and GHdeficiency. Sera from these patients also showed staining of monoamine and GABA nerve terminals in the pituitary intermediate lobe, in agreement with immunoreactivity towards enzymes involved in the biosynthesis of neurotransmittors. By screening of the human pituitary cDNA expression library we identified TDR136 as a major autoantigen in APS 1.
The aim of this study was to evaluate the occurrence of pituitary autoantibodies in patients with nonadenomatous pituitary disease and also to identify novel pituitary autoantigens. Autoantibodies to human pituitary cytosolic proteins were determined by immunoblotting. Reactivity to a M, 49 000 pituitary autoantigen was significantly more frequent in patients with idiopathic pituitary hormone deficiency (6/21 (28%) p< 0.05) as well as their relatives (10/35 (28%) p<0.02) compared to control subjects (3/44 (6.8%)). The importance of these pituitary autoantibodies as markers for LyH remains to be confirmed.
Autoantibodies against human pituitary cytosol and ten additional organspecific autoantigens were measured in sera from 30 patients with empty sella syndrome (ESS). None of the autoantibodies tested was more frequently found in ESS patients compared to healthy controls. Thus, by analysing autoantibodies we did not find evidence of ESS being associated with any autoimmune disease.
Autoantibodies to a novel 36-kDa pituitary cytosolic autoantigen were more common in patients with ACTH-deficiency (12/65 (18.5%) compared to control subjects (2/57 3.5%) (p<0.0214). In addition, autoantibodies to thyroglobulin (M) were positively correlated to immunoreactivity against the 36-kDa pituitary autoantigen.
A human pituitary cDNA expression library was successfully constructed. Immunoscreening identified secretogranin II as a pituitary autoantigen in a patient with partial pituitary insufficiency and empty sella.
By immunohistochemistry, autoantibodies against ACTH and gonadotrophs were detected in sera from patients with autoimmune polyendocrine syndrome type 1 (APS1) and GHdeficiency. Sera from these patients also showed staining of monoamine and GABA nerve terminals in the pituitary intermediate lobe, in agreement with immunoreactivity towards enzymes involved in the biosynthesis of neurotransmittors. By screening of the human pituitary cDNA expression library we identified TDR136 as a major autoantigen in APS 1.
List of papers:
I. Stromberg S, Crock P, Lernmark A, Hulting AL (1998). Pituitary autoantibodies in patients with hypopituitarism and their relatives. J Endocrinol. 157(3): 475-80.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Bensing S, Rorsman F, Crock P, Sanjeevi C, Ericson K, Kampe O, Brismar K, Hulting AL (2004). No evidence for autoimmunity as a major cause of the empty sella syndrome. Exp Clin Endocrinol Diabetes. 112(5): 231-5.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Bensing S, Kasperlik-Zaluska AA, Czarnocka B, Crock PA, Hulting A (2005). Autoantibodies against pituitary proteins in patients with adrenocorticotropin-deficiency. Eur J Clin Invest. 35(2): 126-32.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Bensing S, Hulting AL, Hogg A, Ericson K, Kampe O (2005). Lymphocytic hypophysitis: Report of two biopsy-proven cases and one suspected case with pituitary auotantibodies. [Submitted]
View record in Web of Science®
V. Bensing S, Fetissov S, Perheentupa J, Gustafsson J, Husebye ES, Oscarson M, Ekwall O, Crock PA, Hokfelt T, Hulting AL, Kampe O (2005). Pituitary autoantibodies in autoimmune polyendocrine syndrome type 1. [Submitted]
View record in Web of Science®
I. Stromberg S, Crock P, Lernmark A, Hulting AL (1998). Pituitary autoantibodies in patients with hypopituitarism and their relatives. J Endocrinol. 157(3): 475-80.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Bensing S, Rorsman F, Crock P, Sanjeevi C, Ericson K, Kampe O, Brismar K, Hulting AL (2004). No evidence for autoimmunity as a major cause of the empty sella syndrome. Exp Clin Endocrinol Diabetes. 112(5): 231-5.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Bensing S, Kasperlik-Zaluska AA, Czarnocka B, Crock PA, Hulting A (2005). Autoantibodies against pituitary proteins in patients with adrenocorticotropin-deficiency. Eur J Clin Invest. 35(2): 126-32.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Bensing S, Hulting AL, Hogg A, Ericson K, Kampe O (2005). Lymphocytic hypophysitis: Report of two biopsy-proven cases and one suspected case with pituitary auotantibodies. [Submitted]
View record in Web of Science®
V. Bensing S, Fetissov S, Perheentupa J, Gustafsson J, Husebye ES, Oscarson M, Ekwall O, Crock PA, Hokfelt T, Hulting AL, Kampe O (2005). Pituitary autoantibodies in autoimmune polyendocrine syndrome type 1. [Submitted]
View record in Web of Science®
Issue date: 2005-09-30
Rights:
Publication year: 2005
ISBN: 91-7140-478-3
Statistics
Total Visits
Views | |
---|---|
Pituitary ...(legacy) | 866 |
Pituitary ... | 148 |
Total Visits Per Month
February 2024 | March 2024 | April 2024 | May 2024 | June 2024 | July 2024 | August 2024 | |
---|---|---|---|---|---|---|---|
Pituitary ... | 0 | 0 | 0 | 1 | 4 | 2 | 2 |
File Visits
Views | |
---|---|
thesis.pdf(legacy) | 394 |
thesis.pdf | 195 |
thesis.pdf.txt(legacy) | 2 |
Top country views
Views | |
---|---|
United States | 386 |
Sweden | 85 |
China | 70 |
Germany | 65 |
Russia | 20 |
South Korea | 17 |
Denmark | 13 |
United Kingdom | 13 |
Finland | 9 |
Japan | 8 |
Top cities views
Views | |
---|---|
Ashburn | 41 |
Beijing | 35 |
Romeo | 32 |
Sunnyvale | 27 |
Stockholm | 19 |
Kiez | 18 |
Seoul | 17 |
Nürnberg | 11 |
London | 7 |
Uppsala | 7 |