A three dimensional scaffold for single staged tissue engineering

Abstract

Study I. Aims: Evaluate a collagen-PCL scaffold with minced bladder mucosa in vitro. M&M: Minced tissue was cultured on top of the scaffold. Scaffold properties were evaluated. Results: Good proliferation. Multilayered epithelium after 4 weeks. High tensile strength. Conclusions: Transplant with favorable properties for reconstruction of urogenital tract in one single-staged surgery.

Study II. Aims: Evaluate a collage-PLGA scaffold with minced bladder mucosa in vitro. M&M: Minced tissue was cultured on top and inside the scaffold. Scaffold properties were evaluated. Results: Good proliferation. Multilayered epithelium after 4 weeks. High tensile strength. Conclusions: Transplant with favorable properties for reconstruction of urogenital tract in one single-staged surgery.

Study III. Aims: Evaluate differentiation of bone marrow MSCs into urothelium, separately or on top of a collagen-PCL scaffold. M&M: MSCs were co-cultured with urothelium or cultured with conditioned medium. MSCs were also differentiated on the scaffold. Results: MSCs differentiated into urothelial-like cells after 14 days with both methods, and on top of the collagen-PCL scaffold. Conclusions: In the future, autologous bone marrow MSCs may be a source for urogenital regenerative medicine in cases with lack of native urothelial cells.

Study IV. Aim: Evaluate a collagen-PCL scaffold with minced skin in a rat model. M&M: Minced skin was cultured on top of a collagen-PCL scaffold in vitro and in vivo, in a subcutaneous rat model. Scaffold properties were evaluated. Results: Good integration of scaffold. Keratinocyte proliferation on top of the scaffold that kept its tensile strength and elasticity. Conclusions: Cell expansion on top of the scaffold could take place after transplantation in vivo. This may facilitate future urogenital reconstruction and autologous tissue expansion without in vitro cell culturing.