Muscle function in the critically ill : clinical and experimental investigations
Author: Ahlbeck, Karsten
Date: 2011-09-09
Location: Nanna Svartz föreläsningssal, Karolinska Universitetssjukhuset Solna
Time: 09.00
Department: Inst för fysiologi och farmakologi / Dept of Physiology and Pharmacology
Abstract
It is common that critically ill patients develop muscle weakness in the intensive care unit (ICU), not only delaying mobilisation and increasing the risk of co-morbidities, but also prolonging rehabilitation after hospital care. The aim of this thesis was to describe the diagnosis, time course and possible risk factors for this weakness.
When specific diseases such as CNS lesions, intoxication or other nerve and muscle disorders have been excluded in the ICU, a ”critical illness polyneuropathy and myopathy” (CIPNM) should be considered. The pathology behind this entity is unclear; among possible etiologic factors sepsis, corticosteroids and neuromuscular blocking agents (NMBAs) have been suggested.
CIPNM consists of a nerve pathology (neuropathy) and/or a muscle pathology (myopathy) and is diagnosed by a clinical assessment in combination with neurophysiological examination. The latter can be cumbersome due to the challenging environment in the ICU and is in itself not a definitive method of differentiating between a polyneuropathy and a myopathy.
We demonstrate a rapid method of electrophoresis, using an ultra-thin gel to evaluate the myosin to actin (M/A) ratio as a means of diagnosing critical illness myopathy (CIM). Using this diagnostic tool, there was a significant difference in M/A ratio between the patients having CIM, a control group, and patients having axonal neuropathies.
To evaluate the prevalence of CIPNM and the temporal pattern of its two major components critical illness polyneuropathy (CIP) and CIM, a prospective study was conducted including ICU patients who had been mechanically ventilated for at least 72 hours. The eventual prevalence of CIPNM was investigated, including neurophysiological and clinical examination. Muscle biopsies were obtained, in order to study the myosin to actin ratio and mitochondrial function. All septic patients, who were also receiving corticosteroid treatment, had a CIPNM diagnosis, whereas none of the non-septic patients fulfilled the necessary criteria. As a marker of oxidative stress, mitochondrial superoxide dismutase was increased in all patients, with a marked elevation in the CIPNM group.
To examine possible predisposing risk factors and mechanisms behind CIPNM in an experimental porcine ICU model over 5 days, groups were separated by interventions including corticosteroids, neuromuscular blocking agents and endotoxin, during mechanical ventilation. No group had a pathologic M/A ratio. All groups had significant changes in compound muscle action potential amplitude, including the inactivity/mechanical ventilation only group. The groups including corticosteroid treatment, endotoxin and the combination of all interventions had decreased muscle specific force and mitochondrial complex I activity, which were not seen in the mechanical ventilation group.
In conclusion, this thesis demonstrates an alternative method of diagnosing a critical illness myopathy, which could prove to be both time-efficient and reliable. In ICU patients there was a high prevalence of CIPNM in patients mechanically ventilated for more than 72 hours. An experimental model showed both decreased specific muscle force and mitochondrial complex I activity in intervention groups receiving corticosteroids, endotoxin or a combination, for both respiratory and non-respiratory muscles.
When specific diseases such as CNS lesions, intoxication or other nerve and muscle disorders have been excluded in the ICU, a ”critical illness polyneuropathy and myopathy” (CIPNM) should be considered. The pathology behind this entity is unclear; among possible etiologic factors sepsis, corticosteroids and neuromuscular blocking agents (NMBAs) have been suggested.
CIPNM consists of a nerve pathology (neuropathy) and/or a muscle pathology (myopathy) and is diagnosed by a clinical assessment in combination with neurophysiological examination. The latter can be cumbersome due to the challenging environment in the ICU and is in itself not a definitive method of differentiating between a polyneuropathy and a myopathy.
We demonstrate a rapid method of electrophoresis, using an ultra-thin gel to evaluate the myosin to actin (M/A) ratio as a means of diagnosing critical illness myopathy (CIM). Using this diagnostic tool, there was a significant difference in M/A ratio between the patients having CIM, a control group, and patients having axonal neuropathies.
To evaluate the prevalence of CIPNM and the temporal pattern of its two major components critical illness polyneuropathy (CIP) and CIM, a prospective study was conducted including ICU patients who had been mechanically ventilated for at least 72 hours. The eventual prevalence of CIPNM was investigated, including neurophysiological and clinical examination. Muscle biopsies were obtained, in order to study the myosin to actin ratio and mitochondrial function. All septic patients, who were also receiving corticosteroid treatment, had a CIPNM diagnosis, whereas none of the non-septic patients fulfilled the necessary criteria. As a marker of oxidative stress, mitochondrial superoxide dismutase was increased in all patients, with a marked elevation in the CIPNM group.
To examine possible predisposing risk factors and mechanisms behind CIPNM in an experimental porcine ICU model over 5 days, groups were separated by interventions including corticosteroids, neuromuscular blocking agents and endotoxin, during mechanical ventilation. No group had a pathologic M/A ratio. All groups had significant changes in compound muscle action potential amplitude, including the inactivity/mechanical ventilation only group. The groups including corticosteroid treatment, endotoxin and the combination of all interventions had decreased muscle specific force and mitochondrial complex I activity, which were not seen in the mechanical ventilation group.
In conclusion, this thesis demonstrates an alternative method of diagnosing a critical illness myopathy, which could prove to be both time-efficient and reliable. In ICU patients there was a high prevalence of CIPNM in patients mechanically ventilated for more than 72 hours. An experimental model showed both decreased specific muscle force and mitochondrial complex I activity in intervention groups receiving corticosteroids, endotoxin or a combination, for both respiratory and non-respiratory muscles.
List of papers:
I. Stibler H, Edström L, Ahlbeck K, Remahl S, Ansved T. Electrophoretic determination of the myosin/actin ratio in the diagnosis of critical illness myopathy. Intensive Care Med. 2003; 29: 1515-1528.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Ahlbeck K, Fredriksson K, Rooyackers O, Måbäck G, Remahl S, Ansved T, Eriksson L, Radell P. Signs of critical illness polyneuropathy and myopathy can be seen early in the ICU course. Acta Anaesthesiol Scand. 2009; 53: 717-723.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Ochala J, Ahlbeck K, Radell P, Eriksson LI, Larsson L. Factors underlying the early limb muscle weakness in acute quadriplegic myopathy using an experimental ICU porcine model. PLoS ONE. 2011; 6: e20876.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Ahlbeck K, Fredriksson K, Rooyackers O, Remahl S, Ansved T, Eriksson LI, Radell P. Mitochondrial enzyme activity in respiratory and non-respiratory muscles in a 5-day porcine ICU model. [Manuscript]
I. Stibler H, Edström L, Ahlbeck K, Remahl S, Ansved T. Electrophoretic determination of the myosin/actin ratio in the diagnosis of critical illness myopathy. Intensive Care Med. 2003; 29: 1515-1528.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Ahlbeck K, Fredriksson K, Rooyackers O, Måbäck G, Remahl S, Ansved T, Eriksson L, Radell P. Signs of critical illness polyneuropathy and myopathy can be seen early in the ICU course. Acta Anaesthesiol Scand. 2009; 53: 717-723.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Ochala J, Ahlbeck K, Radell P, Eriksson LI, Larsson L. Factors underlying the early limb muscle weakness in acute quadriplegic myopathy using an experimental ICU porcine model. PLoS ONE. 2011; 6: e20876.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Ahlbeck K, Fredriksson K, Rooyackers O, Remahl S, Ansved T, Eriksson LI, Radell P. Mitochondrial enzyme activity in respiratory and non-respiratory muscles in a 5-day porcine ICU model. [Manuscript]
Institution: Karolinska Institutet
Supervisor: Radell, Peter
Issue date: 2011-08-19
Rights:
Publication year: 2011
ISBN: 978-91-7457-416-6
Statistics
Total Visits
Views | |
---|---|
Muscle ...(legacy) | 768 |
Muscle ... | 196 |
Total Visits Per Month
October 2023 | November 2023 | December 2023 | January 2024 | February 2024 | March 2024 | April 2024 | |
---|---|---|---|---|---|---|---|
Muscle ... | 1 | 1 | 0 | 2 | 4 | 2 | 2 |
File Visits
Views | |
---|---|
Thesis_Karsten_Ahlbeck.pdf(legacy) | 443 |
Spikblad_Karsten_Ahlbeck.pdf(legacy) | 250 |
Thesis_Karsten_Ahlbeck.pdf | 193 |
Spikblad_Karsten_Ahlbeck.pdf | 55 |
Karsten_Ahlbeck_ Thesis.pdf.txt(legacy) | 2 |
Karsten_Ahlbeck_Spikblad.pdf.txt(legacy) | 2 |
Top country views
Views | |
---|---|
United States | 429 |
Sweden | 128 |
Germany | 68 |
China | 46 |
South Korea | 20 |
Denmark | 15 |
United Kingdom | 15 |
Hong Kong | 8 |
Ireland | 8 |
Finland | 7 |
Top cities views
Views | |
---|---|
Ashburn | 50 |
Romeo | 45 |
Sunnyvale | 28 |
Stockholm | 25 |
Kiez | 22 |
Seoul | 19 |
Ballerup | 13 |
Beijing | 10 |
London | 8 |
Dublin | 7 |