Mitochondria and its role in metabolic regulation and skeletal muscle function in healthy and disease conditions
Author: Liu, Zhengye
Date: 2022-02-23
Location: Eva and Georg Klein Lecture Hall, Biomedicum, Karolinska Institutet, Stockholm
Time: 13.00
Department: Inst för fysiologi och farmakologi / Dept of Physiology and Pharmacology
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Thesis (977.4Kb)
Abstract
Skeletal muscle function is critical for our overall health and to be able to perform daily activities. Skeletal muscle has the ability to adapt to various stimuli and mitochondria are known to play an important role in these adaptation processes. Healthy mitochondria are essential for providing skeletal muscle with energy, which are used for various biochemical reactions including generating force and maintaining muscle mass, whereas dysfunctional mitochondria have been associated with loss of skeletal muscle mass and function.
In study I, we investigated the role of nuclear-encoded mitochondrial protein NDUFA4L2 in skeletal muscle. NDUFA4L2 has been shown to decrease oxidative phosphorylation and the production of reactive oxygen species in various tissues and cell lines. We ectopically expressed NDUFA4L2 in mouse skeletal muscles with adenovirus-mediated expression and in vivo electroporation. We found that ectopic NDUFA4L2 expression in skeletal muscle reduced mitochondrial respiration and reactive oxygen species production, together with lowered levels of AMP, ADP, ATP, and NAD+, while the overall protein content of mitochondrial remained unchanged. Furthermore, ectopic expression of NDUFA4L2 resulted in smaller muscle mass and hence weaker muscles. The loss of muscle mass was associated with the activation of atrogenes MurF1 and Mul1, and apoptotic genes caspase 3. We used unilateral femoral artery ligation (FAL) as a mouse model of peripheral vascular disease (PVD) to induce muscle ischemia. Our results showed that NDUFA4L2 was induced in skeletal muscle after FAL. The gene expression of Ndufa4l2 correlated with the reduced capacity of the muscle to produce force.
In study II, we aim to study the role of mitochondria in PVD-induced muscle dysfunction. PVD lowers blood flow to the lower limbs, causing debilitating skeletal muscle myopathy. Interventions that improve distal arterial pressures (i.e., bypass surgery) generally fail to normalize the functional performance of muscle indicating pathophysiological mechanisms inside the skeletal myofibers that reduce overall muscle function. We performed FAL surgery on mice that were fed either a normal chow diet (ND) or a high-fat diet (HFD) for eight weeks. Our results showed that the muscle weakness induced by FAL was exacerbated in mice fed HFD, together with more serious fibrosis and ectopic fat accumulation in these muscles. Our RNA-sequencing results showed that mitochondrial gene expressions had synchronized reduction in ND-FAL legs, while the reduction was attenuated in HFD-FAL legs. Mitochondrial assembly and cellular respiration were identified as the top suppressed pathway in ND-FAL legs, but not in HFD mice. Fibrosis, fat metabolism, and myosin heavy chain isotypes were amongst the top variable genes in control and FAL muscle from normal and obese mice. Inference of proportions of different cell types with ImmuCC found that HFD has already induced an inflammatory response in skeletal muscle without FAL. Our results suggested that mitochondria content and function may be potential targets to improve muscle function in PVD associated with T2D.
Insulin resistance and defects in mitochondrial oxidative phosphorylation (OXPHOS) have been suggested to play an important role in the metabolic dysfunction and muscle impairments caused by T2D. However, we are currently lacking effective treatment against muscle dysfunction in T2D. In study III, we manipulated the mitochondrial electron transport chain (ETC) with our novel NDUFA4L2 genetically knocked-out mouse model. Skeletal muscle lacking NDUFA4L2 appeared stronger, more fatigue resistant, and exhibited higher capillary density and whole-body glucose clearance. NDUFA4L2 knockout mice showed a different metabolic status compared with wild-type litters. Our results indicated that NDUFA4L2 influences skeletal muscle function and hence may be a novel target for T2D-associated muscle dysfunction.
The coactivator PGC-1α1 is pivotal to the regulation of mitochondrial function and content in skeletal muscle. In skeletal muscle after exercise, PGC-1α1 enhanced the expression of kynurenine aminotransferases (Kats), an enzyme that catalyzes the conversion from kynurenine to kynurenic acid. In study IV, we observed that PGC-1α1 increased the expression of genes associated with glycolysis and malate-aspartate shuttle (MAS), together with an elevation in aspartate and glutamate levels. These processes promote energy utilization and facilitate the transfer of electrons from the donors to mitochondrial respiration. Thus, trained skeletal muscle can use kynurenine metabolism to increase the bioenergetic efficiency of glucose oxidation through this PGC-1α1-dependent mechanism. Inhibition of Kat with carbidopa resulted in impairments in aspartate biosynthesis, mitochondrial respiration, and skeletal muscle function. After all, the activate MAS and kynurenine catabolism in skeletal muscle after exercise by PGC-1α1 is important for the muscle’s adaptation to endurance training.
Taken together, these four studies presented in this thesis highlighted the important role of mitochondria in skeletal muscle and the feasibility of targeting mitochondria for the improvement of skeletal muscle function in both healthy and diseased conditions.
In study I, we investigated the role of nuclear-encoded mitochondrial protein NDUFA4L2 in skeletal muscle. NDUFA4L2 has been shown to decrease oxidative phosphorylation and the production of reactive oxygen species in various tissues and cell lines. We ectopically expressed NDUFA4L2 in mouse skeletal muscles with adenovirus-mediated expression and in vivo electroporation. We found that ectopic NDUFA4L2 expression in skeletal muscle reduced mitochondrial respiration and reactive oxygen species production, together with lowered levels of AMP, ADP, ATP, and NAD+, while the overall protein content of mitochondrial remained unchanged. Furthermore, ectopic expression of NDUFA4L2 resulted in smaller muscle mass and hence weaker muscles. The loss of muscle mass was associated with the activation of atrogenes MurF1 and Mul1, and apoptotic genes caspase 3. We used unilateral femoral artery ligation (FAL) as a mouse model of peripheral vascular disease (PVD) to induce muscle ischemia. Our results showed that NDUFA4L2 was induced in skeletal muscle after FAL. The gene expression of Ndufa4l2 correlated with the reduced capacity of the muscle to produce force.
In study II, we aim to study the role of mitochondria in PVD-induced muscle dysfunction. PVD lowers blood flow to the lower limbs, causing debilitating skeletal muscle myopathy. Interventions that improve distal arterial pressures (i.e., bypass surgery) generally fail to normalize the functional performance of muscle indicating pathophysiological mechanisms inside the skeletal myofibers that reduce overall muscle function. We performed FAL surgery on mice that were fed either a normal chow diet (ND) or a high-fat diet (HFD) for eight weeks. Our results showed that the muscle weakness induced by FAL was exacerbated in mice fed HFD, together with more serious fibrosis and ectopic fat accumulation in these muscles. Our RNA-sequencing results showed that mitochondrial gene expressions had synchronized reduction in ND-FAL legs, while the reduction was attenuated in HFD-FAL legs. Mitochondrial assembly and cellular respiration were identified as the top suppressed pathway in ND-FAL legs, but not in HFD mice. Fibrosis, fat metabolism, and myosin heavy chain isotypes were amongst the top variable genes in control and FAL muscle from normal and obese mice. Inference of proportions of different cell types with ImmuCC found that HFD has already induced an inflammatory response in skeletal muscle without FAL. Our results suggested that mitochondria content and function may be potential targets to improve muscle function in PVD associated with T2D.
Insulin resistance and defects in mitochondrial oxidative phosphorylation (OXPHOS) have been suggested to play an important role in the metabolic dysfunction and muscle impairments caused by T2D. However, we are currently lacking effective treatment against muscle dysfunction in T2D. In study III, we manipulated the mitochondrial electron transport chain (ETC) with our novel NDUFA4L2 genetically knocked-out mouse model. Skeletal muscle lacking NDUFA4L2 appeared stronger, more fatigue resistant, and exhibited higher capillary density and whole-body glucose clearance. NDUFA4L2 knockout mice showed a different metabolic status compared with wild-type litters. Our results indicated that NDUFA4L2 influences skeletal muscle function and hence may be a novel target for T2D-associated muscle dysfunction.
The coactivator PGC-1α1 is pivotal to the regulation of mitochondrial function and content in skeletal muscle. In skeletal muscle after exercise, PGC-1α1 enhanced the expression of kynurenine aminotransferases (Kats), an enzyme that catalyzes the conversion from kynurenine to kynurenic acid. In study IV, we observed that PGC-1α1 increased the expression of genes associated with glycolysis and malate-aspartate shuttle (MAS), together with an elevation in aspartate and glutamate levels. These processes promote energy utilization and facilitate the transfer of electrons from the donors to mitochondrial respiration. Thus, trained skeletal muscle can use kynurenine metabolism to increase the bioenergetic efficiency of glucose oxidation through this PGC-1α1-dependent mechanism. Inhibition of Kat with carbidopa resulted in impairments in aspartate biosynthesis, mitochondrial respiration, and skeletal muscle function. After all, the activate MAS and kynurenine catabolism in skeletal muscle after exercise by PGC-1α1 is important for the muscle’s adaptation to endurance training.
Taken together, these four studies presented in this thesis highlighted the important role of mitochondria in skeletal muscle and the feasibility of targeting mitochondria for the improvement of skeletal muscle function in both healthy and diseased conditions.
List of papers:
I. Liu Z*, Chaillou T*, Santos Alves E, Mader T, Jude B, Ferreira DMS, Hynynen H, Cheng AJ, Jonsson WO, Pironti G, Andersson DC, Kenne E, Ruas JL, Tavi P, Lanner JT. Mitochondrial NDUFA4L2 is a novel regulator of skeletal muscle mass and force. FASEB J. 2021 Dec;35(12):e22010. *Equal contribution.
Fulltext (DOI)
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II. Liu Z, Chaillou T, Jude B, Mader T, Mous D, Lanner JT. Skeletal muscle remodeling is response to peripheral artery disease in normal and obese mice. [Manuscript]
III. Liu Z, Santos Alves E, Borg M, Pettersson AM, Zierath J, Lanner JT. Role of mitochondrial protein NDUFA4L2 in skeletal muscle under diseased and healthy condition. [Manuscript]
IV. Agudelo LZ, Ferreira DMS, Dadvar S, Cervenka I, Ketscher L, Izadi M, Zhengye L, Furrer R, Handschin C, Venckunas T, Brazaitis M, Kamandulis S, Lanner JT, Ruas JL. Skeletal muscle PGC-1α1 reroutes kynurenine metabolism to increase energy efficiency and fatigue-resistance. Nat Commun. 2019 Jun 24;10(1):2767.
Fulltext (DOI)
Pubmed
View record in Web of Science®
I. Liu Z*, Chaillou T*, Santos Alves E, Mader T, Jude B, Ferreira DMS, Hynynen H, Cheng AJ, Jonsson WO, Pironti G, Andersson DC, Kenne E, Ruas JL, Tavi P, Lanner JT. Mitochondrial NDUFA4L2 is a novel regulator of skeletal muscle mass and force. FASEB J. 2021 Dec;35(12):e22010. *Equal contribution.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Liu Z, Chaillou T, Jude B, Mader T, Mous D, Lanner JT. Skeletal muscle remodeling is response to peripheral artery disease in normal and obese mice. [Manuscript]
III. Liu Z, Santos Alves E, Borg M, Pettersson AM, Zierath J, Lanner JT. Role of mitochondrial protein NDUFA4L2 in skeletal muscle under diseased and healthy condition. [Manuscript]
IV. Agudelo LZ, Ferreira DMS, Dadvar S, Cervenka I, Ketscher L, Izadi M, Zhengye L, Furrer R, Handschin C, Venckunas T, Brazaitis M, Kamandulis S, Lanner JT, Ruas JL. Skeletal muscle PGC-1α1 reroutes kynurenine metabolism to increase energy efficiency and fatigue-resistance. Nat Commun. 2019 Jun 24;10(1):2767.
Fulltext (DOI)
Pubmed
View record in Web of Science®
Institution: Karolinska Institutet
Supervisor: Lanner, Johanna
Co-supervisor: Ruas, Jorge; Kenne, Ellinor
Issue date: 2022-02-02
Rights:
Publication year: 2022
ISBN: 978-91-8016-521-1
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