Tissue- and site-specific effects of PPARγ activation and its role in chronic inflammation
Author: Pott, Sebastian
Date: 2011-05-31
Location: Föreläsningssalen Petrén, Nobelsväg 12B, Karolinska Institutet, Solna
Time: 09.00
Department: Inst för mikrobiologi, tumör- och cellbiologi / Dept of Microbiology, Tumor and Cell Biology
Abstract
Developmental programming, metabolism, immune function and tissue homeostasis in multicellular organisms are regulated by a plethora of stimuli. Transcription factors (TFs) convert incoming signals to appropriate transcriptional responses of the genome. Nuclear receptors (NRs), a particular family of TFs, are especially well suited for this task given their capacity to influence gene regulation in multiple tissues and conditions. In this work, I addressed how environmental cues affect the expression of NRs and how gene regulation is mediated in different tissues.
My thesis comprises a set of four separate manuscripts of which the first paper established how an important environmental factor, the gut microbiome, modulates NRs, including the peroxisome proliferator-activated receptor gamma (PPARγ), in vivo. The ligand-activated TF PPARγ is a key regulator of adipogenesis and glucose homeostasis, and possesses profound anti-inflammatory properties. In the subsequent manuscripts (paper II-IV), I have used PPARγ as a model to gain insights into the mechanisms that guide tissue-specific activity of NRs on a genome-wide level. To this end I have identified PPARγ binding sites in a genome- wide manner using chromatin immunoprecipitation combined with high-throughput sequencing (ChIP-seq) in adipocytes, macrophages and intestinal epithelium. These data were superimposed with information from gene-expression profiling to facilitate identification of direct PPARγ target genes.
Specifically, our comprehensive genomic analysis of PPARγ binding during adipogenesis confirmed the role of PPARγ as key regulator of this process and, potentially, revealed novel adipogenic target genes of PPARγ (paper II). In addition, an inter-species comparison of PPARγ binding sites in human and mouse macrophages identified a core set of conserved PPARγ targets. In this study we also identified PU.1 as a co-factor necessary for PPARγ function in macrophages (paper III). In the final manuscript, I mapped the genomic landscape of PPARγ-DNA interactions in intestinal epithelial cells (paper IV). These studies revealed that PPARγ antagonizes the WNT/TCF4 signaling pathways potentially identifying a mechanism by which activation of PPARγ affects cell fate of intestinal epithelial cells.
My PhD work yielded important novel insights into general mechanisms related to PPARγ-dependent gene regulation. While in the tissues studied, PPARγ activation seemed to always induce a core set of lipid metabolic genes, tissue-specific utilization of PPARγ binding sites appeared to be dependent on cell type restricted transcription factors which may determine binding site accessibility at the chromatin level.
My studies further suggest that the regulation of lipid metabolism is the evolutionary most conserved function of PPARγ and additional functions might have developed later, representing adaptations to changing metabolic needs and environmental challenges. While not studied in depth, our data on tissue-specific mechanisms of TF binding might also have implications for the interpretation of population-wide genetic studies.
In conclusion, my work has revealed common principles that guide PPARγ activation in a tissue-dependent and -independent manner and has laid the fundament for further detailed molecular studies of NRs in general and PPARγ in particular.
My thesis comprises a set of four separate manuscripts of which the first paper established how an important environmental factor, the gut microbiome, modulates NRs, including the peroxisome proliferator-activated receptor gamma (PPARγ), in vivo. The ligand-activated TF PPARγ is a key regulator of adipogenesis and glucose homeostasis, and possesses profound anti-inflammatory properties. In the subsequent manuscripts (paper II-IV), I have used PPARγ as a model to gain insights into the mechanisms that guide tissue-specific activity of NRs on a genome-wide level. To this end I have identified PPARγ binding sites in a genome- wide manner using chromatin immunoprecipitation combined with high-throughput sequencing (ChIP-seq) in adipocytes, macrophages and intestinal epithelium. These data were superimposed with information from gene-expression profiling to facilitate identification of direct PPARγ target genes.
Specifically, our comprehensive genomic analysis of PPARγ binding during adipogenesis confirmed the role of PPARγ as key regulator of this process and, potentially, revealed novel adipogenic target genes of PPARγ (paper II). In addition, an inter-species comparison of PPARγ binding sites in human and mouse macrophages identified a core set of conserved PPARγ targets. In this study we also identified PU.1 as a co-factor necessary for PPARγ function in macrophages (paper III). In the final manuscript, I mapped the genomic landscape of PPARγ-DNA interactions in intestinal epithelial cells (paper IV). These studies revealed that PPARγ antagonizes the WNT/TCF4 signaling pathways potentially identifying a mechanism by which activation of PPARγ affects cell fate of intestinal epithelial cells.
My PhD work yielded important novel insights into general mechanisms related to PPARγ-dependent gene regulation. While in the tissues studied, PPARγ activation seemed to always induce a core set of lipid metabolic genes, tissue-specific utilization of PPARγ binding sites appeared to be dependent on cell type restricted transcription factors which may determine binding site accessibility at the chromatin level.
My studies further suggest that the regulation of lipid metabolism is the evolutionary most conserved function of PPARγ and additional functions might have developed later, representing adaptations to changing metabolic needs and environmental challenges. While not studied in depth, our data on tissue-specific mechanisms of TF binding might also have implications for the interpretation of population-wide genetic studies.
In conclusion, my work has revealed common principles that guide PPARγ activation in a tissue-dependent and -independent manner and has laid the fundament for further detailed molecular studies of NRs in general and PPARγ in particular.
List of papers:
I. Lundin A, Bok CM, Aronsson L, Björkholm B, Gustafsson JA, Pott S, Arulampalam V, Hibberd M, Rafter J, Pettersson S. Gut flora, Toll-like receptors and nuclear receptors: a tripartite communication that tunes innate immunity in large intestine. Cell Microbiol. (2008) 10(5):1093-103.
Fulltext (DOI)
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II. Hamza MS, Pott S*, Vega VB, Thomsen JS, Kandhadayar GS, Ng PW, Chiu KP, Pettersson S, Wei CL, Ruan Y, Liu ET. De-novo identification of PPARamma/RXR binding sites and direct targets during adipogenesis. PLoS One. (2009) 4(3):e4907.
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III. Pott S, Kamrani NK, Bourque G, Pettersson S, Liu ET. Interplay between PPARG and PU.1 during evolution shape divergent PPARG binding landscapes in human and mouse. [Manuscript]
IV. Pott S, Kamrani NK, Liu ET, Pettersson S. Integrative genomic analysis of PPARγ activity suggests broad antagonism of WNT/β-catenin signaling in intestinal epithelial cells. [Manuscript]
I. Lundin A, Bok CM, Aronsson L, Björkholm B, Gustafsson JA, Pott S, Arulampalam V, Hibberd M, Rafter J, Pettersson S. Gut flora, Toll-like receptors and nuclear receptors: a tripartite communication that tunes innate immunity in large intestine. Cell Microbiol. (2008) 10(5):1093-103.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Hamza MS, Pott S*, Vega VB, Thomsen JS, Kandhadayar GS, Ng PW, Chiu KP, Pettersson S, Wei CL, Ruan Y, Liu ET. De-novo identification of PPARamma/RXR binding sites and direct targets during adipogenesis. PLoS One. (2009) 4(3):e4907.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Pott S, Kamrani NK, Bourque G, Pettersson S, Liu ET. Interplay between PPARG and PU.1 during evolution shape divergent PPARG binding landscapes in human and mouse. [Manuscript]
IV. Pott S, Kamrani NK, Liu ET, Pettersson S. Integrative genomic analysis of PPARγ activity suggests broad antagonism of WNT/β-catenin signaling in intestinal epithelial cells. [Manuscript]
Institution: Karolinska Institutet
Issue date: 2011-05-09
Rights:
Publication year: 2011
ISBN: 978-91-7457-343-5
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