Nitric oxide and eicosanoids : significance and interactions during antigen-induced responses in peripheral lung tissue
Author: Larsson, Anna-Karin
Date: 2007-04-27
Location: Atriumsalen, Nobels väg 12B, Karolinska Institutet
Time: 09.00
Department: Institutet för miljömedicin (IMM) / Institute of Enviromental Medicine
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thesis.pdf (988.8Kb)
Abstract
Asthma means difficulty in breathing and is described as a chronic,
inflammatory disorder that produces narrowing of the lower respiratory
tract. The allergen-induced asthmatic bronchoconstriction is primarily
caused by an IgE-mediated release of the mast cell mediators, histamine
and eicosanoids (leukotrienes and prostanoids). Asthmatics have elevated
levels of nitric oxide (NO) in the exhaled air, which been proposed as a
sign of airway inflammation. In the airways, mast cells represent a major
source of NO. This formed NO may act in an autocrine fashion to suppress
mast cell function, including release of histamine and leukotriene
synthesis, and thereby be a regulator of allergen-induced responses.
Nevertheless, the function of NO in the peripheral lung is not clear.
The aim of this thesis was therefore to establish the role of nitric oxide and eicosanoids during early allergic airway responses in the peripheral lung. Antigen-induced contractions to the allergen ovalbumin were studied in the lung parenchyma obtained from actively sensitized guinea pigs, an in vitro-model for mast cell driven antigen-induced contractions. The peripheral lung is a complex tissue with airway smooth muscle, bronchioles, vessels and connective tissue. To further understand and characterize the contractile responses obtained to allergen or agonists in lung parenchymal tissue, studies in guinea pig precision cut lung slices (GP PCLS) were established and performed. Another aim of this thesis was also to compare species differences during the early allergic airway response in the PCLS.
Inhibition of nitric oxide synthase (NOS) enhanced the contractions to cumulative doses of ovalbumin, whereas addition of the different NO donors SNP and NCX 2057 attenuated the antigen-induced contractions. The action of NO was however not relaxation of airway smooth muscle, since NO potently dilated precontracted vascular preparations and weakly relaxed precontracted tracheal rings, while there was no effect on precontracted GPLP. Instead, NO act as inhibitor of allergen-induced mediator release in the peripheral lung. Inhibition of endogenous NO increased the release of leukotrienes, whereas SNP and NCX 2057 distinctly inhibited the release of histamine or leukotrienes during antigen challenge. In conclusion, the findings support that endogenous NO has a protective role in the peripheral lung as a beneficial immunomodulator of the early allergic airway response. The findings also indicate that different NO donors may have selective and protective anti-inflammatory effects in the peripheral lung tissue.
The GP PCLS was established and represents now a new in vitro model to simultaneously measure airway and vascular responses under cell culture conditions. The study showed that the pharmacology of the guinea pig PCLS most closely resembled that of the corresponding human tissues. In guinea pigs and humans, leukotrienes and prostanoids were primary mediators of the antigen-induced bronchoconstriction. In contrast, the contractile response to antigen in rat PCLS was mainly mediated by serotonin and modulated by locally formed prostanoids, in particular COX-2 derived PGE2, acting at EP1 receptors. Thus, mechanisms by which eicosanoids contribute to the early allergic airway response differ among species.
The aim of this thesis was therefore to establish the role of nitric oxide and eicosanoids during early allergic airway responses in the peripheral lung. Antigen-induced contractions to the allergen ovalbumin were studied in the lung parenchyma obtained from actively sensitized guinea pigs, an in vitro-model for mast cell driven antigen-induced contractions. The peripheral lung is a complex tissue with airway smooth muscle, bronchioles, vessels and connective tissue. To further understand and characterize the contractile responses obtained to allergen or agonists in lung parenchymal tissue, studies in guinea pig precision cut lung slices (GP PCLS) were established and performed. Another aim of this thesis was also to compare species differences during the early allergic airway response in the PCLS.
Inhibition of nitric oxide synthase (NOS) enhanced the contractions to cumulative doses of ovalbumin, whereas addition of the different NO donors SNP and NCX 2057 attenuated the antigen-induced contractions. The action of NO was however not relaxation of airway smooth muscle, since NO potently dilated precontracted vascular preparations and weakly relaxed precontracted tracheal rings, while there was no effect on precontracted GPLP. Instead, NO act as inhibitor of allergen-induced mediator release in the peripheral lung. Inhibition of endogenous NO increased the release of leukotrienes, whereas SNP and NCX 2057 distinctly inhibited the release of histamine or leukotrienes during antigen challenge. In conclusion, the findings support that endogenous NO has a protective role in the peripheral lung as a beneficial immunomodulator of the early allergic airway response. The findings also indicate that different NO donors may have selective and protective anti-inflammatory effects in the peripheral lung tissue.
The GP PCLS was established and represents now a new in vitro model to simultaneously measure airway and vascular responses under cell culture conditions. The study showed that the pharmacology of the guinea pig PCLS most closely resembled that of the corresponding human tissues. In guinea pigs and humans, leukotrienes and prostanoids were primary mediators of the antigen-induced bronchoconstriction. In contrast, the contractile response to antigen in rat PCLS was mainly mediated by serotonin and modulated by locally formed prostanoids, in particular COX-2 derived PGE2, acting at EP1 receptors. Thus, mechanisms by which eicosanoids contribute to the early allergic airway response differ among species.
List of papers:
I. Larsson AK, Back M, Hjoberg J, Dahlen SE (2005). "Inhibition of nitric-oxide synthase enhances antigen-induced contractions and increases release of cysteinyl-leukotrienes in guinea pig lung parenchyma: nitric oxide as a protective factor." J Pharmacol Exp Ther 315(1): 458-65. Epub 2005 Jul 15
Pubmed
II. Larsson A-K, Bäck M, Lundberg J and Dahlén S-E (2007). "Distinct inhibition of mediator release by two different NO donors reduce antigen-induced contractions in the peripheral lung" (Manuscript)
III. Larsson AK, Fumagalli F, Digennaro A, Andersson M, Lundberg J, Edenius C, Govoni M, Monopoli A, Sala A, Dahlen SE, Folco GC (2007). "A new class of nitric oxide-releasing derivatives of cetirizine; pharmacological profile in vascular and airway smooth muscle preparations." Br J Pharmacol Mar 12: Epub ahead of print
Pubmed
IV. Ressmeyer AR, Larsson AK, Vollmer E, Dahlen SE, Uhlig S, Martin C (2006). "Characterisation of guinea pig precision-cut lung slices: comparison with human tissues." Eur Respir J 28(3): 603-11. Epub 2006 May 31
Pubmed
V. Larsson A-K, Dahlén S-E, Ressmeyer A-R, Uhlig S and Martin C (2007). "Modulation of antigen- and serotonin-induced airway contractions in rat precision cut lung slices by prostaglandin E2." (Manuscript)
I. Larsson AK, Back M, Hjoberg J, Dahlen SE (2005). "Inhibition of nitric-oxide synthase enhances antigen-induced contractions and increases release of cysteinyl-leukotrienes in guinea pig lung parenchyma: nitric oxide as a protective factor." J Pharmacol Exp Ther 315(1): 458-65. Epub 2005 Jul 15
Pubmed
II. Larsson A-K, Bäck M, Lundberg J and Dahlén S-E (2007). "Distinct inhibition of mediator release by two different NO donors reduce antigen-induced contractions in the peripheral lung" (Manuscript)
III. Larsson AK, Fumagalli F, Digennaro A, Andersson M, Lundberg J, Edenius C, Govoni M, Monopoli A, Sala A, Dahlen SE, Folco GC (2007). "A new class of nitric oxide-releasing derivatives of cetirizine; pharmacological profile in vascular and airway smooth muscle preparations." Br J Pharmacol Mar 12: Epub ahead of print
Pubmed
IV. Ressmeyer AR, Larsson AK, Vollmer E, Dahlen SE, Uhlig S, Martin C (2006). "Characterisation of guinea pig precision-cut lung slices: comparison with human tissues." Eur Respir J 28(3): 603-11. Epub 2006 May 31
Pubmed
V. Larsson A-K, Dahlén S-E, Ressmeyer A-R, Uhlig S and Martin C (2007). "Modulation of antigen- and serotonin-induced airway contractions in rat precision cut lung slices by prostaglandin E2." (Manuscript)
Issue date: 2007-04-06
Rights:
Publication year: 2007
ISBN: 978-91-7357-155-5
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