Neutrophil granule proteins : modulators of the immune response
Author: Söhnlein, Oliver
Date: 2008-04-25
Location: Samuelssonsalen, Tomtebodavägen 6.
Time: 09.00
Department: Institutionen för fysiologi och farmakologi / Department of Physiology and Pharmacology
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thesis.pdf (1.451Mb)
Abstract
Polymorphonuclear leukocytes (PMN) are multifunctional cells of the
innate immune system which represent the advance guard in inflammatory
reactions. Via the release of granule proteins the PMN communicates with
the endothelium, monocytes, dendritic cells, and lymphocytes and is
thereby critically involved in the fine tuning of the host defense
system. This thesis aimed at elucidating mechanisms by which the PMN and
its granule proteins recruit, activate, and stimulate monocytes and
macrophages. Moreover, PMN secretory products may contribute to tissue
damage, which is another focus of this project.
Extravasation of PMN to the site of injury or infection precedes the recruitment of monocytes. In the first part of the project we addressed causal connections between these two events. There we demonstrate that PMN deposit heparin-binding protein (HBP) from rapidly mobilized secretory vesicles on the endothelial cell surface. In this location HBP interacts with monocytes rolling along the endothelium. Consequently, monocytes become activated and adhere to the vessel wall in increased numbers. In a second study we applied two in vivo models to elucidate the importance of the primary PMN extravasation for the subsequent recruitment of monocytes. We show that PMN extravasation is crucial for the early recruitment of inflammatory monocytes, a subset of monoytes that has recently been shown to be of high significance in inflammatory reactions. As principal mediators of this effect we identify the PMN granule components LL-37 and HBP which employ monocytic formyl peptide receptors to stimulate recruitment of inflammatory monocytes.
Macrophages are of crucial importance in bacterial clearance. In the second part of the project we investigated the impact of PMN secretion products on the macrophage s capacity to phagocytose IgG-opsonized bacteria. PMN secretion was found to strongly enhance the phagocytic capacity of macrophages and thorough investigation revealed HBP and human neutrophil peptides 1-3 (alpha-defensins) as principal mediators of this effect. Mechanistically, these two proteins induce the release of TNFalpha and IFNgamma from macrophages. These activate the macrophage in an autocrine fashion resulting in upregulation of the Fcgamma receptors CD64 and CD32.
Infection with Streptococcus pyogenes of the M1 serotype may lead to acute lung injury and in the third part of the project we addressed the involvement of PMN secretion products in this response. We demonstrate that depletion of PMN, but not of monocytes, completely abolishes the M1 protein-induced lung damage. The lung damage was restored by injection of PMN secretion into neutropenic mice indicating that secretion products of PMN are principle inducers of lung damage following infection with S. pyogens.
Extravasation of PMN to the site of injury or infection precedes the recruitment of monocytes. In the first part of the project we addressed causal connections between these two events. There we demonstrate that PMN deposit heparin-binding protein (HBP) from rapidly mobilized secretory vesicles on the endothelial cell surface. In this location HBP interacts with monocytes rolling along the endothelium. Consequently, monocytes become activated and adhere to the vessel wall in increased numbers. In a second study we applied two in vivo models to elucidate the importance of the primary PMN extravasation for the subsequent recruitment of monocytes. We show that PMN extravasation is crucial for the early recruitment of inflammatory monocytes, a subset of monoytes that has recently been shown to be of high significance in inflammatory reactions. As principal mediators of this effect we identify the PMN granule components LL-37 and HBP which employ monocytic formyl peptide receptors to stimulate recruitment of inflammatory monocytes.
Macrophages are of crucial importance in bacterial clearance. In the second part of the project we investigated the impact of PMN secretion products on the macrophage s capacity to phagocytose IgG-opsonized bacteria. PMN secretion was found to strongly enhance the phagocytic capacity of macrophages and thorough investigation revealed HBP and human neutrophil peptides 1-3 (alpha-defensins) as principal mediators of this effect. Mechanistically, these two proteins induce the release of TNFalpha and IFNgamma from macrophages. These activate the macrophage in an autocrine fashion resulting in upregulation of the Fcgamma receptors CD64 and CD32.
Infection with Streptococcus pyogenes of the M1 serotype may lead to acute lung injury and in the third part of the project we addressed the involvement of PMN secretion products in this response. We demonstrate that depletion of PMN, but not of monocytes, completely abolishes the M1 protein-induced lung damage. The lung damage was restored by injection of PMN secretion into neutropenic mice indicating that secretion products of PMN are principle inducers of lung damage following infection with S. pyogens.
List of papers:
I. Soehnlein O, Xie X, Ulbrich H, Kenne E, Rotzius P, Flodgaard H, Eriksson EE, Lindbom L (2005). "Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions." J Immunol 174(10): 6399-405
Pubmed
II. Soehnlein O, Zernecke A, Eriksson EE, Rothfuchs AG, Pham CT, Bidzhekov K, Herwald H, Rottenberg ME, Weber C, Lindbom L (2008). "Neutrophil secretion products pave the way for inflammatory monocytes." (Submitted)
III. Soehnlein O, Kenne E, Rotzius P, Eriksson EE, Lindbom L (2008). "Neutrophil secretion products regulate anti-bacterial activity in monocytes and macrophages." Clin Exp Immunol 151(1): 139-45. Epub 2007 Dec 7
Pubmed
IV. Soehnlein O, Kai-Larsen Y, Frithiof R, Sorensen OE, Gombart AF, Koeffler HP, Kenne E, Eriksson EE, Herwald H, Agerberth B, Lindbom L (2008). "Neutrophil-derived HBP and HNP1-3 boost bacterial phagocytosis by macrophages." (Submitted)
V. Påhlman LI, Mörgelin M, Eckert J, Johansson L, Russell W, Riesbeck K, Soehnlein O, Lindbom L, Norrby-Teglund A, Schumann RR, Björck L, Herwald H (2006). "Streptococcal M protein: a multipotent and powerful inducer of inflammation." J Immunol 177(2): 1221-8
Pubmed
VI. Soehnlein O, Oehmcke S, Ma X, Rothfuchs AG, Frithiof R, van Roijen N, Mörgelin M, Herwald H, Lindbom L (2008). "Neutrophil degranulation mediates severe lung damage triggered by streptococcal M1 protein." Eur Respir J Mar 5: Epub ahead of print
Pubmed
I. Soehnlein O, Xie X, Ulbrich H, Kenne E, Rotzius P, Flodgaard H, Eriksson EE, Lindbom L (2005). "Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions." J Immunol 174(10): 6399-405
Pubmed
II. Soehnlein O, Zernecke A, Eriksson EE, Rothfuchs AG, Pham CT, Bidzhekov K, Herwald H, Rottenberg ME, Weber C, Lindbom L (2008). "Neutrophil secretion products pave the way for inflammatory monocytes." (Submitted)
III. Soehnlein O, Kenne E, Rotzius P, Eriksson EE, Lindbom L (2008). "Neutrophil secretion products regulate anti-bacterial activity in monocytes and macrophages." Clin Exp Immunol 151(1): 139-45. Epub 2007 Dec 7
Pubmed
IV. Soehnlein O, Kai-Larsen Y, Frithiof R, Sorensen OE, Gombart AF, Koeffler HP, Kenne E, Eriksson EE, Herwald H, Agerberth B, Lindbom L (2008). "Neutrophil-derived HBP and HNP1-3 boost bacterial phagocytosis by macrophages." (Submitted)
V. Påhlman LI, Mörgelin M, Eckert J, Johansson L, Russell W, Riesbeck K, Soehnlein O, Lindbom L, Norrby-Teglund A, Schumann RR, Björck L, Herwald H (2006). "Streptococcal M protein: a multipotent and powerful inducer of inflammation." J Immunol 177(2): 1221-8
Pubmed
VI. Soehnlein O, Oehmcke S, Ma X, Rothfuchs AG, Frithiof R, van Roijen N, Mörgelin M, Herwald H, Lindbom L (2008). "Neutrophil degranulation mediates severe lung damage triggered by streptococcal M1 protein." Eur Respir J Mar 5: Epub ahead of print
Pubmed
Issue date: 2008-04-04
Rights:
Publication year: 2008
ISBN: 978-91-7357-560-7
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