PCR adjusted cure rates in clinical trials of antimalarial drugs in Africa : influence of extended follow-up and consecutive day blood sampling
Author: Mårtensson, Andreas
Date: 2008-11-15
Location: Welandersalen, Infektionskliniken, Karolinska Universitetssjukhuset, Solna
Time: 10.00
Department: Institutionen för medicin / Department of Medicine
View/ Open:
thesis.pdf (561.0Kb)
Abstract
Malaria treatment guidelines are based primarily on results from clinical
trials. Accurate assessment of drug efficacy in such studies therefore
represents a prerequisite to ensure effective and lifesaving treatment
for children with Plasmodium falciparum infections in Africa. The World
Health Organization standard procedures for in vivo assessment include a
patient follow-up duration of at least 28 days. However, during four
weeks or more after initiation of treatment in high transmission areas in
Africa recurrent infections are inevitable, due to the continuous
exposure to infective mosquito bites. Since microscopy cannot distinguish
parasites that have escaped drug action (treatment failure/recrudescence)
from those arising from new mosquito inoculations (new
infections/reinfections), polymerase chain reaction (PCR) based
genotyping of malaria infections has been introduced to improve this
distinction. PCR adjustment is presently achieved by analysis of two
blood samples, one collected at enrolment and the other at time of
recurrent parasitaemia. However, despite being a sensitive technique PCR
may not identify minority clones in complex malaria infections and
parasites sequestering in the deep vascular system during the later part
of the erythrocytic life cycle.
This thesis primarily addresses two fundamental aspects of the assessment of PCR adjusted efficacy of antimalarial drugs in Africa, i.e. the influence of extended follow-up beyond 28 days and added consecutive day blood sampling.
The results reveal that both an extension of follow-up and consecutive day blood sampling improve the identification of recrudescences. By pooling data from 1414 children enrolled in three studies included in this thesis the overall sensitivity of the 28 day follow-up schedule to identify recrudescences was calculated to 48/72=67% [95% CI 55-76%] compared with day 42 assessment. Moreover, consecutive day, instead of single day blood sampling improved identification of recrudescences by circa 20%.
The work in this thesis also highlights the potential influence of the number of genetic markers used and the choice of DNA extraction method on the efficacy outcome in the clinical trials
Thus, assessment of PCR adjusted cure rates in high endemic areas depends substantially on methodology and should therefore be interpreted with caution. The results from this thesis should be considered in the design of antimalarial drug trials to improve assessment of antimalarial drug efficacy in Africa.
This thesis primarily addresses two fundamental aspects of the assessment of PCR adjusted efficacy of antimalarial drugs in Africa, i.e. the influence of extended follow-up beyond 28 days and added consecutive day blood sampling.
The results reveal that both an extension of follow-up and consecutive day blood sampling improve the identification of recrudescences. By pooling data from 1414 children enrolled in three studies included in this thesis the overall sensitivity of the 28 day follow-up schedule to identify recrudescences was calculated to 48/72=67% [95% CI 55-76%] compared with day 42 assessment. Moreover, consecutive day, instead of single day blood sampling improved identification of recrudescences by circa 20%.
The work in this thesis also highlights the potential influence of the number of genetic markers used and the choice of DNA extraction method on the efficacy outcome in the clinical trials
Thus, assessment of PCR adjusted cure rates in high endemic areas depends substantially on methodology and should therefore be interpreted with caution. The results from this thesis should be considered in the design of antimalarial drug trials to improve assessment of antimalarial drug efficacy in Africa.
List of papers:
I. Mårtensson A, Strömberg J, Sisowath C, Msellem MI, Gil JP, Montgomery SM, Olliaro P, Ali AS, Björkman A. (2005). "Efficacy of artesunate plus amodiaquine versus that of artemether-lumefantrine for the treatment of uncomplicated childhood Plasmodium falciparum malaria in Zanzibar, Tanzania." Clin Infect Dis 41(8): 1079-86.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Abdulla S, Sagara I, Borrmann S, D´Alessandro U, González R, Hamel M, Ogutu B, Mårtensson A, Lyimo J, Maiga H, Sasi P, Nahum A, Bassat Q, Juma E, Otieno L, Björkman A, Beck HP, Andriano K, Cousin M, Lefèvre G, Ubben D, Premji Z (2008). "Efficacy and safety of artemether-lumefantrine dispersible tablets compared with crushed commercial tablets in African infants and children with uncomplicated malaria: a randomised, single-blind, multicentre trial." Lancet. [Accepted]
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Bereczky S, Mårtensson A, Gil JP, Färnert A. (2005). "Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum." Am J Trop Med Hyg 72(3): 249-51.
Pubmed
View record in Web of Science®
IV. Martensson A, Ngasala B, Ursing J, Isabel Veiga M, Wiklund L, Membi C, Montgomery SM, Premji Z, Farnert A, Bjorkman A (2007). "Influence of consecutive-day blood sampling on polymerase chain reaction-adjusted parasitological cure rates in an antimalarial-drug trial conducted in Tanzania." J Infect Dis 195(4): 597-601.
Fulltext (DOI)
Pubmed
View record in Web of Science®
V. Mårtensson A, Carlsson AM, Ngasala B, Dahlström S, Membi C, Musih MA, Omary S, Montgomery SM, Rombo L, Abdullah S, Premji Z, Gil JP, Björkman A (2008). "Plasmodium falciparum population dynamics during the early phase of antimalarial drug treatment in Tanzanian children with acute uncomplicated." [Manuscript]
I. Mårtensson A, Strömberg J, Sisowath C, Msellem MI, Gil JP, Montgomery SM, Olliaro P, Ali AS, Björkman A. (2005). "Efficacy of artesunate plus amodiaquine versus that of artemether-lumefantrine for the treatment of uncomplicated childhood Plasmodium falciparum malaria in Zanzibar, Tanzania." Clin Infect Dis 41(8): 1079-86.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Abdulla S, Sagara I, Borrmann S, D´Alessandro U, González R, Hamel M, Ogutu B, Mårtensson A, Lyimo J, Maiga H, Sasi P, Nahum A, Bassat Q, Juma E, Otieno L, Björkman A, Beck HP, Andriano K, Cousin M, Lefèvre G, Ubben D, Premji Z (2008). "Efficacy and safety of artemether-lumefantrine dispersible tablets compared with crushed commercial tablets in African infants and children with uncomplicated malaria: a randomised, single-blind, multicentre trial." Lancet. [Accepted]
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Bereczky S, Mårtensson A, Gil JP, Färnert A. (2005). "Short report: Rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum." Am J Trop Med Hyg 72(3): 249-51.
Pubmed
View record in Web of Science®
IV. Martensson A, Ngasala B, Ursing J, Isabel Veiga M, Wiklund L, Membi C, Montgomery SM, Premji Z, Farnert A, Bjorkman A (2007). "Influence of consecutive-day blood sampling on polymerase chain reaction-adjusted parasitological cure rates in an antimalarial-drug trial conducted in Tanzania." J Infect Dis 195(4): 597-601.
Fulltext (DOI)
Pubmed
View record in Web of Science®
V. Mårtensson A, Carlsson AM, Ngasala B, Dahlström S, Membi C, Musih MA, Omary S, Montgomery SM, Rombo L, Abdullah S, Premji Z, Gil JP, Björkman A (2008). "Plasmodium falciparum population dynamics during the early phase of antimalarial drug treatment in Tanzanian children with acute uncomplicated." [Manuscript]
Issue date: 2008-10-25
Rights:
Publication year: 2008
ISBN: 978-91-7409-229-5
Statistics
Total Visits
Views | |
---|---|
PCR ...(legacy) | 879 |
PCR ... | 189 |
Total Visits Per Month
October 2023 | November 2023 | December 2023 | January 2024 | February 2024 | March 2024 | April 2024 | |
---|---|---|---|---|---|---|---|
PCR ... | 4 | 0 | 2 | 2 | 0 | 1 | 0 |
File Visits
Views | |
---|---|
thesis.pdf(legacy) | 379 |
thesis.pdf | 375 |
thesis.pdf.txt(legacy) | 2 |
Top country views
Views | |
---|---|
United States | 379 |
Sweden | 95 |
Germany | 84 |
China | 75 |
United Kingdom | 22 |
Iraq | 19 |
South Korea | 16 |
Denmark | 15 |
Finland | 15 |
India | 9 |
Top cities views
Views | |
---|---|
Ashburn | 46 |
Beijing | 39 |
Sunnyvale | 33 |
Kiez | 28 |
Romeo | 26 |
Stockholm | 17 |
Seoul | 14 |
London | 12 |
Nürnberg | 11 |
Ballerup | 9 |