Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics: Recent submissions
Now showing items 41-60 of 318
-
Microglial autophagy-associated phagocytosis is essential for recovery from neuroinflammation
(Inst för klinisk neurovetenskap / Dept of Clinical Neuroscience, 2021-12-14)Multiple sclerosis (MS) is a leading cause of incurable progressive disability in young adults caused by inflammation and neurodegeneration in the central nervous system (CNS). The capacity of microglia to clear tissue ... -
Novel FISH methods to unveil genome architecture
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-11-22)The genome consists of incredibly long DNA strands that encode all the vital information for the cell to function. The DNA inclusion in the very tight nuclear space and, simultaneously, the establishment of a hierarchical ... -
Developing methods for mapping genetic heterogeneity in tumors : from bulk to single-cell resolution
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-11-15)Tumors are complex ecosystems composed of billions of cells that constantly evolve over time. Intra-tumor heterogeneity (ITH) represents the diversity of this complex environment quantifiable both at the genetic and ... -
Analysis of antibodies to cartilage oligomeric matrix protein in rheumatoid arthritis and in mouse models
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-10-13)Rheumatoid arthritis (RA), a common autoimmune disease, affects 0.5-1% of the world’s population. It is not only an important health problem but also a socioeconomic problem considering that it can lead to joint destruction ... -
Redox regulation of T cells in autoimmunity
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-09-03)Autoimmune disorders affect a significant part of the population and therefore present a serious health and economic burden. One of the most common autoimmune diseases is rheumatoid arthritis (RA), affecting 0.5-1% of the ... -
Positional cloning of polymorphic loci that control autoreactive T cells
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-08-13)Autoimmune diseases are expensive diseases, both financially and socially, because they are in most cases life long and require a long-lasting treatment. They can also be life threatening, as they are among top 10 causes ... -
Identifying genetic determinants of T cell-dependent autoimmunity using forward genetics
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-07-29)Rheumatoid arthritis (RA) affects 0.5-1% of the population and is an important health and socioeconomic problem. RA has a high degree of heritability. Thus, extensive efforts have been made to better understand the genetic ... -
Antibodies as pathogenic factors and biomarkers in rheumatoid arthritis
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-05-21)Ever since the evolution of an adaptive immune system capable of creating immune receptors that may recognize self-antigens, we have been at risk of autoimmunity. There are over 100 different types of autoimmune diseases ... -
Plurifaceted proteomics in studying cellular dynamics and action mechanisms of anticancer drugs
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-05-05)Mass spectrometry (MS)-based proteomics has developed tremendously in recent years and was the leading technology for many novel methods to study protein chemistry. Contrary to classical approaches based on Western blot, ... -
Methods development for the investigation of the mammalian genome radial architecture : the quantitative side
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-04-16)The nucleus of mammalian cells cradles the genome, an ensemble of nucleic acid macromolecular polymers that store information in a physical form. For a cell to perform life-sustaining processes, reading and utilizing the ... -
Spatial regulation of membrane receptor signaling using DNA origami
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-04-07)Juxtracrine signaling between apposing membrane receptors and ligands is an important class of intercellular communication. Much focus has been directed towards studying the biochemical interactions between receptors and ... -
Ribosomal RNA dynamics studied by NMR-spectroscopy
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-03-11)The ribosome is a large macromolecular machine that consists of both ribosomal proteins and ribosomal RNA (rRNA). It is a complex consisting of two subunits held together by non-covalent interactions, intersubunit bridges ... -
A DNA-nanoassembly-based approach to map membrane protein nanoenvironments
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-03-05)Most proteins at the plasma membrane are not uniformly distributed but localize to dynamic domains of nanoscale dimensions. To investigate their functional relevance, there is a need for methods that enable comprehensive ... -
Cell states along oligodendrocyte development and disease
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-12-23)The brain, one of the most complex organs in the body, where an immense diversity of cell states emerges from simple structure, where function arises from sets of regulatory principles and pattern persist where individual ... -
Novel methods for mapping genome organization and genome fragility in the 3D space of the nucleus
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-11-13)For over a century, scientists have been trying to understand how the DNA molecule that is so tightly packed in the micro space of the nucleus sustains critical cellular processes such as transcription, replication or the ... -
Conformational dynamics in microRNAs : the example of miR-34a targeting Sirt1 mRNA
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-06-17)In biology, regulatory mechanisms are essential to achieve complex tasks, as virtually every process can be positively or negatively modulated in its outcome, upon different cues. In humans, microRNAs (miRNAs) constitute ... -
X-chromosome upregulation is driven by increased burst frequency
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and BiophysicsInst för cell- och molekylärbiologi / Dept of Cell and Molecular Biology, 2020-05-12)Ohno's hypothesis postulates that X-chromosome upregulation rectifies X-dose imbalance relative to autosomal genes, present in two active copies per cell. Here we dissected X-upregulation into kinetics of transcription, ... -
Studies of the thioredoxin system in redox signaling and oxidative stress
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-05-08)Reactive oxygen species exert reversible posttranslational modifications in proteins containing redox sensitive thiols, thereby affecting several cellular processes and protein functions. Reductive enzyme systems, such as ... -
Applications of genomic tools to decode genome function
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-04-27)The revolution in sequencing technologies has greatly advanced our understanding of genomes. Many regulatory elements lacking protein-coding ability, such as long non-coding RNAs have been identified and characterized in ... -
3D imaging and quantitative analysis of intact tissues and organs
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-04-17)Embryonic development and tumor growth are highly complex and dynamic processes that exist in both time and space. To fully understand the molecular mechanisms that control these processes, it is crucial to study RNA ...