Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics: Recent submissions
Now showing items 21-40 of 317
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Custom-tailored DNA origami mechanics for cellular applications
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2023-02-22)DNA molecules have been used as the building block for the self-assembly of artificial nanostructures. In particular, the DNA origami method has made the design of DNA nanostructures more robust and approachable. Different ... -
Characterization of GTPBPs involved in ribosome assembly and function in human mitochondria
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2023-01-30)Mitochondria are organelles often referred to as the powerhouses of the cell as they provide most of the chemical energy via aerobic respiration. However, in the last half a century, it has become clear that their function ... -
Transcriptomic cellular diversity of the early human developing brain
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2023-01-27)The complexity of the mammalian brain is partly reflected in its cell type diversity which influences the function of neurons that encode the behavior of animals. Brain cell type diversity emerges during embryonic stages, ... -
Development of DNA origami-based tools for cancer treatment
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2023-01-17)DNA has been used as material for the assembly of objects at different scales. Particularly, the introduction of DNA origami has been inspiring the design and construction of many different versions of DNA nanostructures ... -
Illuminating tissue organization by imaging the spatial transcriptome
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-12-19)Our bodies consist of a large collection of cells that each have their own function in the organ that they reside in. The cells are grouped by functionality in cell types that arise during development as the result of the ... -
Chemical proteomics for drug target deconvolution and to study biological systems
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-10-27)Mass spectrometry-based proteomics has become an irreplaceable method for the unbiased and system-wide study of protein chemistry. As modern proteomics methods can not only be applied to quantify protein expression levels, ... -
DNA fragility in the context of neural stem cell fate : a multi-method integrative exploration of genome dynamics
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-10-18)Recent advances in mapping the complex genetic architecture underlying various debilitating brain disorders have enabled identification of several genetic risk variants. However, these risk variants only explain part of ... -
RNA polymerase I inhibition : mechanism and exploitation in cancer treatment
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-10-10)Cancer is an umbrella term for diseases characterized by uncontrollably proliferating abnormal cells that often have also gained the ability to spread and invade other tissues. It is one of the leading causes of death ... -
Exploring the dynamic epigenome in pluripotent stem cells using quantitative methods
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-10-10)A totipotent stem cell has the potential to give rise to the trillions of cells in the human body, all carrying the very same genetic information. Through differentiation events, gene expression changes guided by epigenetic ... -
Nucleic acid tools for detection and characterization of biological systems
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-10-06)Nucleic acids, DNA and RNA, are naturally occurring biopolymers synthetized by cells to store and propagate genetic information. They can be found in eukaryotic cells, bacteria, archaea and viruses and, thanks to the ... -
Transcription kinetics in pluripotent cells : RNA turnover, transcription velocity, and epigenomic regulation
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-09-12)Transcriptional regulation is one of the primary steps in gene expression control. It is now appreciated that a large fraction of coding genome is transcribed in concert of other functional RNAs. A quantitative method for ... -
Fighting isoaspartate
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-09-02)Isoaspartate (IsoAsp) is a damaging amino acid residue generated either from asparagine (Asn) deamidation or aspartate (Asp) isomerization. Both reactions are spontaneous in physiological conditions and require no enzymes. ... -
Quantitative methods for profiling dynamic chromatin features
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-05-12)Living systems, from entire organisms down to the single cells constituting them are dynamic entities that continuously adapt and respond to their local environment. Cells achieve this through gene expression programs ... -
Gene regulatory mechanisms in the oligodendrocyte lineage in development and disease
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-05-12)Oligodendrocytes are the myelinating cells of the central nervous system (CNS). They contribute to the neuronal network through the insulation of neuronal axons, facilitating communication between neurons and providing ... -
RNA systems for NMR studies in vitro and in vivo
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-04-05)NMR spectroscopy is an excellent tool to study the structure-function relationship of RNA. Such measurements are usually performed in vitro, which requires large amounts of isotope- labeled sample in high purity and can ... -
DNA G-quadruplex structures in the mammalian genome : dissecting genome-wide mechanisms of formation and turnover of DNA G-quadruplexes
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-03-11)Since the beginning of 20th century, when the G-tetrad was first discovered, scientists have been seeking to understand the role of G4 quadruplex structures (G4s) in replication, DNA damage, telomere integrity and the ... -
Identification of novel modulators of protein synthesis and nucleolar biology using high throughput phenotypic screens
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-02-23)Protein synthesis and ribosome biogenesis are fundamental steps in gene expression and constitute the most energy demanding processes in living cells. Dysregulation of these processes is associated to a variety of human ... -
Developing and democratizing tools for spatially resolved profiling of genome and transcriptome
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-01-21)The common thread of the work presented in this thesis is the development and democratization of tools that facilitate the spatial profiling of genome and transcriptome. Paper I describes iFISH, a resource that ... -
Mitochondrial RNA processing in health and disease
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-12-28)Mitochondria are often described as the powerhouses of the cell, providing the main cellular energy source in the form of adenosine triphosphate. Five enzyme complexes, collectively termed the oxidative phosphorylation ... -
Microglial autophagy-associated phagocytosis is essential for recovery from neuroinflammation
(Inst för klinisk neurovetenskap / Dept of Clinical Neuroscience, 2021-12-14)Multiple sclerosis (MS) is a leading cause of incurable progressive disability in young adults caused by inflammation and neurodegeneration in the central nervous system (CNS). The capacity of microglia to clear tissue ...