Regulation of human pancreas hormone secretion by autonomic innervation

Diabetes mellitus is a silent killer doing away with one person every 10 seconds. We speak of diabetes when the organism cannot control the right level of glucose in the blood. The hormones insulin and glucagon secreted by the islets of Langerhans are the major players maintaining glucose homeostasis. In the living organism, the function of the islets is orchestrated by their interaction with other organs through the vasculature and with the nervous system. Most of our current knowledge of islet biology has been obtained by using mouse models, but caution is needed, as mice are not simply small humans. Indeed, recent studies have revealed that the cell composition and architecture of the human islet are different from that of mouse islets. Thus, other important features such as nervous regulation of islet function may also be different.

The work in this thesis aimed to identify the role of innervation for islet function. Our hypothesis is that autonomic and paracrine signals are involved in islet function and that the relative role of these components varies among species. To identify the sympathetic and parasympathetic components of innervation as well as their cellular targets we used immunohistochemical staining of human and mouse pancreatic sections. In contrast to mouse, human islets are devoid of parasympathetic innervation. Instead, human alpha cells possess the machinery for exocytosis of acetylcholine, the major parasympathetic neurotransmitter. Our findings suggest that human islets depend less on neural cholinergic input than mouse islets. Alpha cells secrete acetylcholine as a paracrine signal priming the human beta cell to respond optimally to subsequent increases in glucose concentration. In addition, noradrenergic fibers contact few endocrine cells in the human islet and preferentially innervate smooth muscle cells of the islet vasculature. This suggests that sympathetic innervation regulates hormone secretion by controlling the blood flow rather than modulating endocrine cell function directly.

By taking advantage of our recently developed noninvasive anterior chamber of the eye imaging platform we were able to study the role of innervation in the maintenance of glucose homeostasis in vivo. We studied the process of reinnervation and revascularization of intraocular islet grafts and showed that islets orchestrate the process of engraftment to restore their original microenvironment. Islet grafts from two different mouse strains and human xenografts showed innervation patterns similar to those in pancreatic sections in situ. Islet grafts displayed the characteristic fenestrae of the pancreatic vascular endothelium independently of the origin of the new vessels. In addition, the model allowed controlling the fraction of the graft vasculature that is contributed by the donor islet endothelial cells to the point that the original donor vasculature of the islet is restored. Recording graft function while manipulating the eye’s neural input through the pupillary light reflex revealed functional differences in parasympathetic innervation between the two mouse strains. The eye platform also allowed us to follow cell dynamics during immune responses, which will enable investigations aimed at clarifying the role of innervation in the pathogenesis of autoimmune diabetes.

To study human islet biology in vivo we further adopted the eye model by transplanting human islets into the eye of diabetic immune compromised mice. Human xenografts reversed diabetes and tightly controlled plasma glucose concentrations. Moreover, our results provided the first real time monitoring of revascularization and blood flow inside human islets and graft function could be modulated by local drug administration. Our findings establish a “humanized” mouse model to investigate human islet biology in vivo that will allow addressing how nervous input affects endocrine function or blood flow in human islets. The physiological relevance of the anterior chamber of the eye model is further underscored by the therapeutic potential as a novel transplantation site to treat type 1 diabetic patients.

List of scientific papers

I. Rayner Rodriguez-Diaz, Midhat H. Abdulreda, Alexander L. Formoso, Itai Gans, Camilo Ricordi, Per-Olof Berggren, Alejandro Caicedo (2011). Autonomic axons in the human Endocrine pancreas show unique innervation patterns. Cell Metabolism. Jul 6;14(1):45-54.
https://doi.org/10.1016/j.cmet.2011.05.008

II. Rayner Rodriguez-Diaz, Robin Dando, M. Caroline Jacques-Silva, Alberto Fachado, Judith Molina, Midhat H. Abdulreda, Camilo Ricordi, Stephen D. Roper, Per-Olof Berggren, Alejandro Caicedo (2011). Acetylcholine is released by human alpha cells as a paracrine signal to prime beta cell insulin secretion. Nat Med. Jul 19;17(7):888-92.
https://doi.org/10.1038/nm.2371

III. Rayner Rodriguez-Diaz, Robin Dando, Y. Anthony Huang, Per-Olof Berggren, Stephen D. Roper, Alejandro Caicedo (2011). Real time detection of acetylcholine release from the human endocrine pancreas. [Submitted]

IV. Rayner Rodriguez-Diaz, Stephan Speier, Damaris Molano, Alexander L. Formoso, Itai Gans, Midhat Abdulreda, Over Cabrera, Alberto Fachado, Camillo Ricordi, Ingo Leibiger, Antonello Pileggi, Per-Olof Berggren, and Alejandro Caicedo (2011). A simple noninvasive system for investigating the role of innervation in pancreatic islet function in vivo. [Submitted]

V. Daniel Nyqvist, Stephan Speier, Rayner Rodriguez-Diaz, R. Damaris Molano, Sasa Lipovsek, Marjam Rupnik, Andrea Dicker, Erwin Ilegems, Elsie Zahr-Akrawi, Judith Molina, Maitee Lopez-Cabeza M, Susana Villate, Midhat H. Abdulreda, Camilo Ricordi, Alejandro Caicedo, Pileggi, Per-Olof Berggren (2011). Donor islet endothelial cells in pancreatic islet revascularization. Diabetes. 60(10):2571-7.
https://doi.org/10.2337/db10-1711

VI. Midhat H. Abdulreda, M, Gaetano Faleo, R. Damaris Molano, Judith Molina, Y. Tan, Ron Echeverria, Elsie Zahr-Akrawi, Rayner Rodriguez-Diaz, P. Edlund, Ingo Leibiger, Alisson L. Bayer, Victor L. Perez, Camilo Ricordi, Alejandro Caicedo, Antonello Pileggi, Per-Olof Berggren (2011). High resolution, non-invasive longitudinal live imaging of immune responses. Proc Natl Acad Sci U S A. 108(31):12863-8.
https://doi.org/10.1073/pnas.1105002108

VII. Victor L. Perez, Alejandro Caicedo, Dora M. Berman, E. Arrieta, Midhat H. Abdulreda, Rayner Rodriguez-Diaz, Antonello Pileggi, E. Hernandez, S. R. Dubovy, Jean-Marie Parel, Camilo Ricordi, Norma M. Kenyon, Per-Olof Berggren (2011). The anterior chamber of the eye as a clinical transplantation site for the treatment of diabetes. Diabetologia. 54(5):1121-6.
https://doi.org/10.1007/s00125-011-2091-y