Molecular mechanisms in idiopathic inflammatory myopathies
Background: Myositis is a group of rare autoimmune diseases. Muscle weakness and fatigue are the dominant symptoms and inflammation with T cells and macrophages is a characteristic finding in muscle tissue. Currently high-dose and long-term glucocorticoids is still the most important treatment but with limited efficacy and worrisome side effects. Therefore, investigations regarding inflammatory mediators and their roles in myositis pathogenesis are important in order to develop new therapies.
Methods: In order to investigate the roles of different inflammatory mediators in patients with myositis, biological samples were investigated from clinically well-characterized patients in different phases of disease. Several techniques were employed: immunohistochemical staining on muscle tissue and cultured skeletal muscle cells; flow cytometry, ELISA and chemiluminescence immunoassay on blood samples. Hypotheses regarding molecular mechanisms for muscle weakness and fatigue were tested in animal models, where we mainly used enzymatically dissociated muscle fibers and mechanically dissected muscle fibers to measure the force and calcium release from the sarcoplasmic reticulum under various defined molecular conditions.
Results: Based on previous observations on IL-1 expression in muscle tissue we first tested the role of IL-1 by using IL-1 blockade, anakinra, for 12 months in patients with refractory myositis. Eight of 15 patients had a clinical response which correlated to some response in biomarkers in blood, but muscle tissue inflammation persisted. Therefore, we searched for a new immune modulating target and found IL-15 to be expressed in muscle tissue of patients with myositis, and higher IL-15 expression was associated with more muscle dysfunction. Another approach was to test for the role of inflammatory molecules in early phases of muscle inflammation. In this context the extra nuclear presence of the alarmin high-mobility group box 1 (HMGB1) in the muscle fibers of patients with myositis without detectable inflammatory infiltrates is of interest. By in vitro experiments we showed that HMGB1 can influence muscle function by accelerating muscle fatigue and inducing MHC-class I expression via TLR4. Another receptor for HMGB1, TLR2, was also found in muscle tissue of patients with myositis and our animal study demonstrated that by knocking out TLR2 the skeletal muscle fibers became more muscle fatigue resistant.
Conclusion: Collectively, the investigations in my thesis suggest that inflammatory mediators play important roles in the pathogenesis of myositis and different molecules may contribute in different phases of disease. Hereby, HMGB1 might induce muscle dysfunction at an early stage of the disease via TLR4 (or/and TLR2). IL-15 could be involved in developing muscle dysfunction via maintaining T cell homeostasis in the muscles. IL-1 may be important in a subset of patients by driving the adaptive immune system. However, more studies are needed for a comprehensive understanding of their roles in order to develop new therapies.
List of scientific papers
I. Anakinra treatment in patients with refractory inflammatory myopathies and possible predictive response biomarkers. Mei Zong, Christina Dorph, Maryam Dastmalchi, Helene Alexanderson, Jennifer Pieper, Petra Amoudruz, Sevim Barbasso Helmers, Inger Nennesmo, Vivianne Malmström, Ingrid E. Lundberg. Ann Rheum Dis. 2014 May 1; 73(5):913-20.
https://doi.org/10.1136/annrheumdis-2012-202857
II. Effects of conventional immunosuppressive treatment on Interleukin-15 in muscle tissue of patients with polymyositis or dermatomyositis. Mei Zong, Ingela Loell, Eva Lindroos, Gustavo Nader, Helene Alexanderson, Christina Ståhl Hallengren, Kristian Borg, Snjolaug Arnardottir, Iain B Mclnnes and Ingrid Lundberg. Ann Rheum Dis. 2012 Jun; 71(6):1055-63
https://doi.org/10.1136/annrheumdis-2011-200495
III. TLR4 as receptor for HMGB1 induced muscle dysfunction in myositis. Mei Zong, Joseph D. Bruton, Cecilia Grundtman, Huan Yang, Jian Hua Li, Helene Alexanderson, Karin Palmblad, Ulf Andersson, Helena E. Harris, Ingrid E. Lundberg and Håkan Westerblad. Ann Rheum Dis. 2013 Aug; 72(8):1390-9.
https://doi.org/10.1136/annrheumdis-2012-202207
IV. Loss of TLR2 increases fatigue resistance in mouse skeletal muscles Mei Zong, Arthur Cheng, Abram Katz, Niklas Ivarsson, Ingrid E. Lundberg, Håkan Westerblad, Helena E. Harris, Joseph D. Bruton. [Submitted]
History
Defence date
2014-10-10Department
- Department of Medicine, Solna
Publisher/Institution
Karolinska InstitutetMain supervisor
Lundberg, IngridPublication year
2014Thesis type
- Doctoral thesis
ISBN
978-91-7549-618-4Number of supporting papers
4Language
- eng