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Glutaredoxin 3 from Escherichia coli : NMR structure analysis and structural aspects of the enzymatic mechanism
The structure of Escherichia coli glutaredoxin 3 (Grx3) was investigated using NMR spectroscopy in combination with isotope labeling. Nearly complete sequence specific assignment were obtained for the reduced Grx3 using a 15N enriched protein sample. The secondary structure and topology of reduced Grx3 was shown to be similar to another glutaredoxin in E. coli, Grx1, based on the analysis of chemical shifts, coupling constants, amide exchange rates and short and medium range NOEs. In the NMR spectra of reduced Grx3, the thiol proton of the more C-terminal active site cysteine, Cys14, is present despite presaturation of the solvent resonance, with a chemical shift of 7.6 ppm. The large downfield shift and the fact that the thiol proton is observed in the NMR spectra suggests that it is hydrogen bonded and protected from exchange with solvent.
Additionally, the sequence specific assignments for reduced, unfolded Grx3 at pH 3.5 were obtained using a combination of a 3D 15N-HSQC-(TOCSY-NOESY)-15N-HSQC and a 3D-ROESY-HSQC experiment. The former makes use of the relatively large chemical shift dispersion of the 15N chemical shift in both indirect dimensions to overcome chemical shift degeneracies present in the unfolded protein. Analysis of coupling constants and NOEs, combined with a comparison between the observed chemical shifts and published values for random coil chemical shifts, indicate that Grx3 is practically completely unfolded at this pH.
A technique for producing fractionally [13C]-enriched protein was developed, using an algal hydrolysate as the sole carbon source. The fractional enrichment was utilized in an HSQC experiment for the determination of one-bond coupling constants, 1JHC. Accurate 1JHC couplings of C[alpha]H groups contain information on the dihedral angle [psi], and are thus potentially useful constraints for NMR structure determinations.
The NMR structure of the Grx3-glutathione mixed disulfide (Grx3-SG) was determined using a Grx3 C14S-C65Y mutant. The Grx3-SG complex is an intermediate in the enzymatic reaction and the C14S mutation was needed to trap the intermediate. The structure was calculated with the program DYANA and energy minimized in explicit water using the program OPAL. The RMSD of protein backbone atoms (N, C[alpha], C') in a final set of 20 conformers was 0.57 Å relative to the mean structure. The interactions between Grx3 and glutathione were defined by 21 intermolecular distance constraints, and the glutathione was found to bind in an anti-parallel ß bridge to Va152 of Grx3. The structure also suggests the presence of a second glutathione binding site which could explain the observed specificity for reduction of glutaredoxin-glutathione mixed disulfides by glutathione.
Except for the active site, the NMR spectra of reduced Grx3 and Grx3-SG are very similar. A comparison of chemical shifts and coupling constants in the different spectra led to a proposal of the interactions stabilizing the Cys11 thiolate in reduced Grx3, which were further supported by activity measurements for a number of Grx3 mutants.
History
Defence date
1998-11-20Department
- Department of Medical Biochemistry and Biophysics
Publication year
1998Thesis type
- Doctoral thesis
ISBN-10
91-628-3251-4Language
- eng