A three dimensional scaffold for single staged tissue engineering

Study I. Aims: Evaluate a collagen-PCL scaffold with minced bladder mucosa in vitro. M&M: Minced tissue was cultured on top of the scaffold. Scaffold properties were evaluated. Results: Good proliferation. Multilayered epithelium after 4 weeks. High tensile strength. Conclusions: Transplant with favorable properties for reconstruction of urogenital tract in one single-staged surgery.

Study II. Aims: Evaluate a collage-PLGA scaffold with minced bladder mucosa in vitro. M&M: Minced tissue was cultured on top and inside the scaffold. Scaffold properties were evaluated. Results: Good proliferation. Multilayered epithelium after 4 weeks. High tensile strength. Conclusions: Transplant with favorable properties for reconstruction of urogenital tract in one single-staged surgery.

Study III. Aims: Evaluate differentiation of bone marrow MSCs into urothelium, separately or on top of a collagen-PCL scaffold. M&M: MSCs were co-cultured with urothelium or cultured with conditioned medium. MSCs were also differentiated on the scaffold. Results: MSCs differentiated into urothelial-like cells after 14 days with both methods, and on top of the collagen-PCL scaffold. Conclusions: In the future, autologous bone marrow MSCs may be a source for urogenital regenerative medicine in cases with lack of native urothelial cells.

Study IV. Aim: Evaluate a collagen-PCL scaffold with minced skin in a rat model. M&M: Minced skin was cultured on top of a collagen-PCL scaffold in vitro and in vivo, in a subcutaneous rat model. Scaffold properties were evaluated. Results: Good integration of scaffold. Keratinocyte proliferation on top of the scaffold that kept its tensile strength and elasticity. Conclusions: Cell expansion on top of the scaffold could take place after transplantation in vivo. This may facilitate future urogenital reconstruction and autologous tissue expansion without in vitro cell culturing.

List of scientific papers

I. Fatemeh Ajalloueian, Said Zeiai, Ramiro Rojas, Magdalena Fossum, Jöns Hilborn. One-stage tissue engineering of bladder wall patches for an easy-to-use approach at the surgical table. Tissue Eng Part C. 2013, 19(9), 688-96.
https://doi.org/10.1089/ten.tec.2012.0633

II. Fatemeh Ajalloueian, Said Zeiai, Magdalena Fossum, Jöns G Hilborn. Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue expansion. Biomaterials. 2014, 35(22), 5741-8.
https://doi.org/10.1016/j.biomaterials.2014.04.002

III. Jixue Zhao, Said Zeiai, Åsa Ekblad, Agneta Nordenskjöld, Jöns G Hilborn, Cecilia Götherström, Magdalena Fossum. Transdifferentiation of autologous bone marrow cells on a collagen-poly( ɛ-caprolactone) scaffold for tissue engineering in complete lack of native urothelium. J R Soc Interface. 2014, 11(96).
https://doi.org/10.1098/rsif.2014.0233

IV. Said Zeiai, Clara I Chamorro, Jinxing Huo, Jöns G Hilborn, Magdalena Fossum. The concept of In vivo tissue engineering as a single surgical procedure. [Manuscript]