A three dimensional scaffold for single staged tissue engineering

<p>Study I. Aims: Evaluate a collagen-PCL scaffold with minced bladder mucosa in vitro. M&M: Minced tissue was cultured on top of the scaffold. Scaffold properties were evaluated. Results: Good proliferation. Multilayered epithelium after 4 weeks. High tensile strength. Conclusions: Transplant with favorable properties for reconstruction of urogenital tract in one single-staged surgery.</p><p>Study II. Aims: Evaluate a collage-PLGA scaffold with minced bladder mucosa in vitro. M&M: Minced tissue was cultured on top and inside the scaffold. Scaffold properties were evaluated. Results: Good proliferation. Multilayered epithelium after 4 weeks. High tensile strength. Conclusions: Transplant with favorable properties for reconstruction of urogenital tract in one single-staged surgery.</p><p>Study III. Aims: Evaluate differentiation of bone marrow MSCs into urothelium, separately or on top of a collagen-PCL scaffold. M&M: MSCs were co-cultured with urothelium or cultured with conditioned medium. MSCs were also differentiated on the scaffold. Results: MSCs differentiated into urothelial-like cells after 14 days with both methods, and on top of the collagen-PCL scaffold. Conclusions: In the future, autologous bone marrow MSCs may be a source for urogenital regenerative medicine in cases with lack of native urothelial cells.</p><p>Study IV. Aim: Evaluate a collagen-PCL scaffold with minced skin in a rat model. M&M: Minced skin was cultured on top of a collagen-PCL scaffold in vitro and in vivo, in a subcutaneous rat model. Scaffold properties were evaluated. Results: Good integration of scaffold. Keratinocyte proliferation on top of the scaffold that kept its tensile strength and elasticity. Conclusions: Cell expansion on top of the scaffold could take place after transplantation in vivo. This may facilitate future urogenital reconstruction and autologous tissue expansion without in vitro cell culturing.</p><h3>List of scientific papers</h3><p>I. Fatemeh Ajalloueian, Said Zeiai, Ramiro Rojas, Magdalena Fossum, Jöns Hilborn. One-stage tissue engineering of bladder wall patches for an easy-to-use approach at the surgical table. Tissue Eng Part C. 2013, 19(9), 688-96. <br><a href="https://doi.org/10.1089/ten.tec.2012.0633">https://doi.org/10.1089/ten.tec.2012.0633</a><br><br> </p><p>II. Fatemeh Ajalloueian, Said Zeiai, Magdalena Fossum, Jöns G Hilborn. Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue expansion. Biomaterials. 2014, 35(22), 5741-8. <br><a href="https://doi.org/10.1016/j.biomaterials.2014.04.002">https://doi.org/10.1016/j.biomaterials.2014.04.002</a><br><br> </p><p>III. Jixue Zhao, Said Zeiai, Åsa Ekblad, Agneta Nordenskjöld, Jöns G Hilborn, Cecilia Götherström, Magdalena Fossum. Transdifferentiation of autologous bone marrow cells on a collagen-poly( ɛ-caprolactone) scaffold for tissue engineering in complete lack of native urothelium. J R Soc Interface. 2014, 11(96). <br><a href="https://doi.org/10.1098/rsif.2014.0233">https://doi.org/10.1098/rsif.2014.0233</a><br><br> </p><p>IV. Said Zeiai, Clara I Chamorro, Jinxing Huo, Jöns G Hilborn, Magdalena Fossum. The concept of In vivo tissue engineering as a single surgical procedure. [Manuscript]</p>