Selenocompounds in leukemia treatment : advantages and pitfalls
Author: Zheng, Wenyi
Date: 2020-02-21
Location: Svartsjön NOVUM, Blickagången 6, Karolinska Universitetssjukhuset Huddinge
Time: 09.30
Department: Inst för laboratoriemedicin / Dept of Laboratory Medicine
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Thesis (4.675Mb)
Abstract
In 2018, cancer was reported to be the second top cause of morbidity and mortality globally with over 9 million deaths. Hematological malignancies including leukemia constitute about 7% of the total cancer cases. Substantial developments in treatment modalities and strategies have increased the 5-year survival rate in leukemia to around 60% in developed countries. However, complete remission and long-term disease control are not yet achieved. Oxidative stress is imprinted in many types of cancer including leukemia and represents a valuable trait for achieving leukemiaselective cytotoxicity. The present thesis represents a systematic study of the role of redox-active selenocompounds (SeCs) in leukemia treatment.
Six diverse selenocompounds representing different compound classes were studied. Among them, p-xyelenselenocyanate (p-XSC) was shown to have the most potent cytotoxic activity against several leukemia cell lines carrying distinct oncogenes. p-XSC exerted its cytotoxicity in a concentration- and time-dependent manner. Mechanistic studies revealed that the cytotoxicity of p-XSC was mediated by upregulation of oxidative stress and accompanied with massive mitochondria damages. Importantly, the cytotoxicity of SeCs was antagonized by albumin which is ubiquitously present in biological conditions. By the combination of two distinct, but complementary selenium speciation methods including liquid chromatographymass spectrometry and X-ray absorption spectroscopy, we showed that cytotoxic SeCs were capable of transforming into selenol intermediates that subsequently bound to albumin via selenium-sulfur bond. Furthermore, we found that the macromolecular selenocompound-albumin conjugate was also internalized and able to kill leukemia cells. In addition to interfering with cytotoxicity, binding of SeCs to albumin also hindered the quantification of these compounds in biological matrix e.g. plasma. To elucidate the pharmacokinetics properties of SeCs for in vivo applications, we developed a novel REductive Cleavage and Instant Derivatization (RECID) method, by which we were able to measure both free and albumin-bound SeCs. In the leukemia mouse model, intravenous administration of p-XSC was shown to reduce the disease burden in whole body as well as in bone marrow.
In conclusion, the results obtained in the present thesis provide substantial experimental evidences that redox-active SeCs, in particular p-XSC, possess high therapeutic potential as treatment for leukemia. Further investigations to optimize treatment regimen and to design an appropriate drug carrier are needed to achieve successful clinical trials.
Six diverse selenocompounds representing different compound classes were studied. Among them, p-xyelenselenocyanate (p-XSC) was shown to have the most potent cytotoxic activity against several leukemia cell lines carrying distinct oncogenes. p-XSC exerted its cytotoxicity in a concentration- and time-dependent manner. Mechanistic studies revealed that the cytotoxicity of p-XSC was mediated by upregulation of oxidative stress and accompanied with massive mitochondria damages. Importantly, the cytotoxicity of SeCs was antagonized by albumin which is ubiquitously present in biological conditions. By the combination of two distinct, but complementary selenium speciation methods including liquid chromatographymass spectrometry and X-ray absorption spectroscopy, we showed that cytotoxic SeCs were capable of transforming into selenol intermediates that subsequently bound to albumin via selenium-sulfur bond. Furthermore, we found that the macromolecular selenocompound-albumin conjugate was also internalized and able to kill leukemia cells. In addition to interfering with cytotoxicity, binding of SeCs to albumin also hindered the quantification of these compounds in biological matrix e.g. plasma. To elucidate the pharmacokinetics properties of SeCs for in vivo applications, we developed a novel REductive Cleavage and Instant Derivatization (RECID) method, by which we were able to measure both free and albumin-bound SeCs. In the leukemia mouse model, intravenous administration of p-XSC was shown to reduce the disease burden in whole body as well as in bone marrow.
In conclusion, the results obtained in the present thesis provide substantial experimental evidences that redox-active SeCs, in particular p-XSC, possess high therapeutic potential as treatment for leukemia. Further investigations to optimize treatment regimen and to design an appropriate drug carrier are needed to achieve successful clinical trials.
List of papers:
I. Wenyi Zheng#, Ying Zhao#, Rui He, Dhanu Gupta, Samir El-Andaloussi, Manuchehr Abedi-Valugerdi, Manuel Valiente, Moustapha Hassan. Utilization of redox-active selenocompound for leukemia treatment in vitro and in vivo. #Equal contribution. [Manuscript]
II. Wenyi Zheng, Fadwa Benkessou, Brigitte Twelkmeyer, Siyao Wang, Tobias Ginman, Håkan Ottosson, Manuchehr Abedi-Valugerdi, Maria Angels Subirana, Ying Zhao, and Moustapha Hassan. Rapid and robust quantification of p-xyleneselenocyanate in plasma via derivatization. Analytical Chemistry. 2017, 89: 7586-7592.
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III. Wenyi Zheng, Rui He, Roberto Boada, Maria Angels Subirana, Tobias Ginman, Håkan Ottosson, Manuel Valiente, Moustapha Hassan. A general covalent binding model between cytotoxic selenocompounds and albumin revealed by mass spectrometry and X-ray absorption spectroscopy. [Accepted]
Fulltext (DOI)
Pubmed
IV. Wenyi Zheng, Roberto Boada, Rui He, Tingting Xiao, Fei Ye, Laura Simonelli, Manuel Valiente, Ying Zhao Moustapha Hassan. Extracellular albumin covalently sequesters selenocompounds and determines cytotoxicity. International Journal of Molecular Sciences. 2019, 20: 4734-4746.
Fulltext (DOI)
Pubmed
View record in Web of Science®
I. Wenyi Zheng#, Ying Zhao#, Rui He, Dhanu Gupta, Samir El-Andaloussi, Manuchehr Abedi-Valugerdi, Manuel Valiente, Moustapha Hassan. Utilization of redox-active selenocompound for leukemia treatment in vitro and in vivo. #Equal contribution. [Manuscript]
II. Wenyi Zheng, Fadwa Benkessou, Brigitte Twelkmeyer, Siyao Wang, Tobias Ginman, Håkan Ottosson, Manuchehr Abedi-Valugerdi, Maria Angels Subirana, Ying Zhao, and Moustapha Hassan. Rapid and robust quantification of p-xyleneselenocyanate in plasma via derivatization. Analytical Chemistry. 2017, 89: 7586-7592.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Wenyi Zheng, Rui He, Roberto Boada, Maria Angels Subirana, Tobias Ginman, Håkan Ottosson, Manuel Valiente, Moustapha Hassan. A general covalent binding model between cytotoxic selenocompounds and albumin revealed by mass spectrometry and X-ray absorption spectroscopy. [Accepted]
Fulltext (DOI)
Pubmed
IV. Wenyi Zheng, Roberto Boada, Rui He, Tingting Xiao, Fei Ye, Laura Simonelli, Manuel Valiente, Ying Zhao Moustapha Hassan. Extracellular albumin covalently sequesters selenocompounds and determines cytotoxicity. International Journal of Molecular Sciences. 2019, 20: 4734-4746.
Fulltext (DOI)
Pubmed
View record in Web of Science®
Institution: Karolinska Institutet
Supervisor: Hassan, Moustapha
Co-supervisor: Zhao, Ying; Abedi-Valugerdi, Manuchehr
Issue date: 2020-01-27
Rights:
Publication year: 2020
ISBN: 978-91-7831-638-0
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