Intracellular mechanisms of skeletal muscle fatigue : role of creatine kinase
Author: Dahlstedt, Anders
Date: 2001-12-14
Location: Föreläsningssalen Cesar, Berzeliuslaboratoriet, Karolinska Institutet
Time: 9.00
Department: Institutionen för fysiologi och farmakologi / Department of Physiology and Pharmacology
Abstract
During intense physical activity the maximal power output of skeletal
muscles is reduced, a condition described as muscle fatigue. The cause of
impaired muscle function seen in fatigue is multifactorial. Creatine
kinase (CK, catalyzes PCr + ADP 4n Cr + ATP) is considered important for
niinimizing changes of [ATP]/[ADP] that may occur during periods of
intense activity. Using knockout mice that lack the gene coding for CK
(CC mice) andlor by inhibiting CK pharmacologically, the role of CK was
studied during skeletal muscle fatigue, both in whole muscles and single
intact muscle fibres.
The maximum shortening velocity (Vo) was reduced during fatigue or with inhibition of CK with 2,4-dinitro-l-fluorobenzene (DNFB). These results are consistent with the idea that an increased ADP reduces Vo during fatigue. Furthermore, both CK-/- and DNFB exposed fibres displayed a transient decline of [Ca2+]i and force early in fatiguing stimulation. This shows that PCr breakdown supports SR Ca2+ release during short term, high-intensity activity. However, during low-intensity fatiguing stimulation, tetanic [Ca2+]i and force are better maintained in fibres with nonoperating CK as compared to control fibres. This indicates that PCr breakdown may actually cause failure of SR Ca2+ release and contribute to fatigue during low-intensity stimulation. To distinguish between possible adaptive changes and direct CK effects in single CC muscle fibres, contractile performance was compared at rest and during high-intensity fatiguing stimulation before and after injection of purified CK.
The results show that the wild-type phenotype is partially restored in CKinjected CK-/- fibres. Fast-twitch muscles of Ck-/--mice provides a good model for. studying the effects of Pi on muscle function, since they have higher Pi at rest and do not accumulate Pi during fatigue. Using this model, all major functional changes observed in early fatigue can be explained by an increased [Pi]; that is, Pi reduces tetanic force and myofibrillar Ca2+ sensitivity, increases tetanic [Ca2+]i, slows force relaxation, and reduces the rate of SR Ca2+ uptake. In conclusion, increased [ADP] and [Pi] are important factors in skeletal muscle fatigue.
The maximum shortening velocity (Vo) was reduced during fatigue or with inhibition of CK with 2,4-dinitro-l-fluorobenzene (DNFB). These results are consistent with the idea that an increased ADP reduces Vo during fatigue. Furthermore, both CK-/- and DNFB exposed fibres displayed a transient decline of [Ca2+]i and force early in fatiguing stimulation. This shows that PCr breakdown supports SR Ca2+ release during short term, high-intensity activity. However, during low-intensity fatiguing stimulation, tetanic [Ca2+]i and force are better maintained in fibres with nonoperating CK as compared to control fibres. This indicates that PCr breakdown may actually cause failure of SR Ca2+ release and contribute to fatigue during low-intensity stimulation. To distinguish between possible adaptive changes and direct CK effects in single CC muscle fibres, contractile performance was compared at rest and during high-intensity fatiguing stimulation before and after injection of purified CK.
The results show that the wild-type phenotype is partially restored in CKinjected CK-/- fibres. Fast-twitch muscles of Ck-/--mice provides a good model for. studying the effects of Pi on muscle function, since they have higher Pi at rest and do not accumulate Pi during fatigue. Using this model, all major functional changes observed in early fatigue can be explained by an increased [Pi]; that is, Pi reduces tetanic force and myofibrillar Ca2+ sensitivity, increases tetanic [Ca2+]i, slows force relaxation, and reduces the rate of SR Ca2+ uptake. In conclusion, increased [ADP] and [Pi] are important factors in skeletal muscle fatigue.
List of papers:
I. Westerblad H, Dahlstedt AJ, Lannergren J (1998). "Mechanisms underlying reduced maximum shortening velocity during fatigue of intact, single fibres of mouse muscle. " J Physiol 510 ( Pt 1): 269-77
Pubmed
II. Dahlstedt AJ, Katz A, Wieringa B, Westerblad H (2000). "Is creatine kinase responsible for fatigue? Studies of isolated skeletal muscle deficient in creatine kinase. " FASEB J 14(7): 982-90
Pubmed
III. Dahlstedt AJ, Katz A, Westerblad H (2001). "Role of myoplasmic phosphate in contractile function of skeletal muscle: studies on creatine kinase-deficient mice. " J Physiol 533(Pt 2): 379-88
Pubmed
IV. Dahlstedt AJ, Westerblad H (2001). "Inhibition of creatine kinase reduces the rate of fatigue-induced decrease in tetanic [Ca(2+)](i) in mouse skeletal muscle. " J Physiol 533(Pt 3): 639-49
Pubmed
V. Dahlstedt AJ, Katz A, Westerblad H (2001). "Creatine kinase (CK) injection partially restores contractile function of CK deficient mouse skeletal muscle cells." (Manuscript)
I. Westerblad H, Dahlstedt AJ, Lannergren J (1998). "Mechanisms underlying reduced maximum shortening velocity during fatigue of intact, single fibres of mouse muscle. " J Physiol 510 ( Pt 1): 269-77
Pubmed
II. Dahlstedt AJ, Katz A, Wieringa B, Westerblad H (2000). "Is creatine kinase responsible for fatigue? Studies of isolated skeletal muscle deficient in creatine kinase. " FASEB J 14(7): 982-90
Pubmed
III. Dahlstedt AJ, Katz A, Westerblad H (2001). "Role of myoplasmic phosphate in contractile function of skeletal muscle: studies on creatine kinase-deficient mice. " J Physiol 533(Pt 2): 379-88
Pubmed
IV. Dahlstedt AJ, Westerblad H (2001). "Inhibition of creatine kinase reduces the rate of fatigue-induced decrease in tetanic [Ca(2+)](i) in mouse skeletal muscle. " J Physiol 533(Pt 3): 639-49
Pubmed
V. Dahlstedt AJ, Katz A, Westerblad H (2001). "Creatine kinase (CK) injection partially restores contractile function of CK deficient mouse skeletal muscle cells." (Manuscript)
Issue date: 2001-11-23
Publication year: 2001
ISBN: 91-631-1673-1
Statistics
Total Visits
Views | |
---|---|
Intracellular ...(legacy) | 317 |
Intracellular ... | 135 |
Total Visits Per Month
October 2023 | November 2023 | December 2023 | January 2024 | February 2024 | March 2024 | April 2024 | |
---|---|---|---|---|---|---|---|
Intracellular ... | 2 | 2 | 11 | 2 | 0 | 1 | 2 |
Top country views
Views | |
---|---|
United States | 82 |
Sweden | 56 |
Netherlands | 48 |
Germany | 45 |
China | 35 |
South Korea | 13 |
Ireland | 9 |
Russia | 9 |
Finland | 6 |
Canada | 4 |
Top cities views
Views | |
---|---|
Schiphol | 20 |
Kiez | 19 |
Seoul | 11 |
Stockholm | 7 |
Beijing | 6 |
Dublin | 6 |
Sunnyvale | 5 |
Bangkok | 4 |
Des Moines | 4 |
Mount Laurel | 4 |