Phenotype and genotype effects on the transcriptome in cardiovascular disease : tools to identify candidate genes
Author: Folkersen, Lasse
Date: 2011-12-16
Location: Rehabsalen, S2, Norrbacka, Karolinska universitetssjukhuset, Solna
Time: 09.00
Department: Inst för medicin, Solna / Dept of Medicine, Solna
Abstract
The overarching purpose of this thesis was to investigate the expression of human genes and how they relate to cardiovascular disease. This was pursued through five papers, each of which investigated different aspects of gene expression.
Paper I improved the technology for extracting gene expression information from microarrays. Its main purpose was to introduce the software package GeneRegionScan, which was developed for analysis of genomic regions with inaccurate annotation.
Paper II explored the genomic region surrounding the first identified single‐ nucleotide polymorphism (SNP) associated with cardiovascular disease. The purpose of the paper was to investigate transcript isoforms of the regional genes and their association to the risk‐SNPs in the region. Eight new transcript isoforms were introduced, but no pattern of association with genotype was observed.
Paper III extended the methods of paper II, by expanding the search to 166 risk‐ SNPs known at the time. For each of these SNPs, it was hypothesized that one or more proximal genes had expression levels that were associated to the genotype of the SNP in question. It was reported that 47 of the SNPs had such genes, thus progressing from risk‐SNPs towards risk‐genes. In addition the paper introduced new concepts on distance between gene and risk SNP and on the tissue‐ specificity of associations.
Paper IV investigated the gene expression pattern of thoracic aortic aneurysm. It had previously been observed that patients with congenital bicuspid aortic valve (BAV) had increased severity and earlier onset age of aneurysm, when compared to patients with the normal tricuspid aortic valve (TAV). A fundamentally differing gene expression profile was observed between these two patient groups, and a possible immunological involvement in TAV patients was reported.
Paper V asked if the high‐throughput methods of genomics and transcriptomics could be applied towards better prediction of future ischemic events in patients with established atherosclerosis. Risk‐SNP profiles, gene expression profiles of circulating blood cells, and gene expression profiles of carotid plaque samples were utilized. Particularly gene expression profiles of carotid plaque provided improved prediction above that of the established risk markers of serum lipids, gender, age and smoking.
Paper I improved the technology for extracting gene expression information from microarrays. Its main purpose was to introduce the software package GeneRegionScan, which was developed for analysis of genomic regions with inaccurate annotation.
Paper II explored the genomic region surrounding the first identified single‐ nucleotide polymorphism (SNP) associated with cardiovascular disease. The purpose of the paper was to investigate transcript isoforms of the regional genes and their association to the risk‐SNPs in the region. Eight new transcript isoforms were introduced, but no pattern of association with genotype was observed.
Paper III extended the methods of paper II, by expanding the search to 166 risk‐ SNPs known at the time. For each of these SNPs, it was hypothesized that one or more proximal genes had expression levels that were associated to the genotype of the SNP in question. It was reported that 47 of the SNPs had such genes, thus progressing from risk‐SNPs towards risk‐genes. In addition the paper introduced new concepts on distance between gene and risk SNP and on the tissue‐ specificity of associations.
Paper IV investigated the gene expression pattern of thoracic aortic aneurysm. It had previously been observed that patients with congenital bicuspid aortic valve (BAV) had increased severity and earlier onset age of aneurysm, when compared to patients with the normal tricuspid aortic valve (TAV). A fundamentally differing gene expression profile was observed between these two patient groups, and a possible immunological involvement in TAV patients was reported.
Paper V asked if the high‐throughput methods of genomics and transcriptomics could be applied towards better prediction of future ischemic events in patients with established atherosclerosis. Risk‐SNP profiles, gene expression profiles of circulating blood cells, and gene expression profiles of carotid plaque samples were utilized. Particularly gene expression profiles of carotid plaque provided improved prediction above that of the established risk markers of serum lipids, gender, age and smoking.
List of papers:
I. Folkersen L, Diez D, Wheelock CE, Haeggström JZ, Goto S, Eriksson P, Gabrielsen A. (2009) GeneRegionScan: a Bioconductor package for probelevel analysis of specific, small regions of the genome. Bioinformatics. Aug 1;25(15):1978-9. Epub 2009 Apr 27.
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II. Folkersen L*, Kyriakou T*, Goel A, Peden J, Mälarstig A, Paulsson-Berne G, Hamsten A, Hugh Watkins, Franco-Cereceda A, Gabrielsen A, Eriksson P. (2009) Relationship between CAD risk genotype in the chromosome 9p21 locus and gene expression. Identification of eight new ANRIL splice variants. PLoS One. Nov 2;4(11):e7677. *Equal contribution.
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III. Folkersen L, van't Hooft F, Chernogubova E, Agardh HE, Hansson GK, Hedin U, Liska J, Syvänen AC, Paulsson-Berne G, Franco-Cereceda A, Hamsten A, Gabrielsen A, Eriksson P. (2010) Association of genetic risk variants with expression of proximal genes identifies novel susceptibility genes for cardiovascular disease. Circ Cardiovasc Genet. Aug;3(4):365-73. Epub 2010 Jun 19.
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IV. Folkersen L, Wågsäter D, Paloschi V, Jackson V, Petrini J, Kurtovic S, Maleki S, Eriksson MJ, Caidahl K, Hamsten A, Michel JB, Liska J, Gabrielsen A, Franco-Cereceda A, Eriksson P. (2011) Unraveling the divergent gene expression profiles in bicuspid and tricuspid aortic valve patients with thoracic aortic dilatation - the ASAP study. Mol Med. Sep 27. [Epub ahead of print]
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V. Lasse Folkersen, Jonas Persson, Johan Ekstrand, Hanna E Agardh, Göran K Hansson, Anders Gabrielsen, Ulf Hedin, and Gabrielle Paulsson-Berne. Prediction of ischemic events based on transcriptomic and genomic profiling in patients undergoing carotid endarterectomy. [Manuscript]
I. Folkersen L, Diez D, Wheelock CE, Haeggström JZ, Goto S, Eriksson P, Gabrielsen A. (2009) GeneRegionScan: a Bioconductor package for probelevel analysis of specific, small regions of the genome. Bioinformatics. Aug 1;25(15):1978-9. Epub 2009 Apr 27.
Fulltext (DOI)
View record in Web of Science®
Pubmed
II. Folkersen L*, Kyriakou T*, Goel A, Peden J, Mälarstig A, Paulsson-Berne G, Hamsten A, Hugh Watkins, Franco-Cereceda A, Gabrielsen A, Eriksson P. (2009) Relationship between CAD risk genotype in the chromosome 9p21 locus and gene expression. Identification of eight new ANRIL splice variants. PLoS One. Nov 2;4(11):e7677. *Equal contribution.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Folkersen L, van't Hooft F, Chernogubova E, Agardh HE, Hansson GK, Hedin U, Liska J, Syvänen AC, Paulsson-Berne G, Franco-Cereceda A, Hamsten A, Gabrielsen A, Eriksson P. (2010) Association of genetic risk variants with expression of proximal genes identifies novel susceptibility genes for cardiovascular disease. Circ Cardiovasc Genet. Aug;3(4):365-73. Epub 2010 Jun 19.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Folkersen L, Wågsäter D, Paloschi V, Jackson V, Petrini J, Kurtovic S, Maleki S, Eriksson MJ, Caidahl K, Hamsten A, Michel JB, Liska J, Gabrielsen A, Franco-Cereceda A, Eriksson P. (2011) Unraveling the divergent gene expression profiles in bicuspid and tricuspid aortic valve patients with thoracic aortic dilatation - the ASAP study. Mol Med. Sep 27. [Epub ahead of print]
Fulltext (DOI)
V. Lasse Folkersen, Jonas Persson, Johan Ekstrand, Hanna E Agardh, Göran K Hansson, Anders Gabrielsen, Ulf Hedin, and Gabrielle Paulsson-Berne. Prediction of ischemic events based on transcriptomic and genomic profiling in patients undergoing carotid endarterectomy. [Manuscript]
Institution: Karolinska Institutet
Supervisor: Gabrielsen, Anders
Issue date: 2011-11-25
Rights:
Publication year: 2011
ISBN: 978-91-7457-584-2
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