Abstract
The focus of the work presented in this thesis has been to study the
structures of the capsular polysaccharide from Serratia marcescens
O14:K12, and the O-antigen polysaccharides from Hafnia alvei strain 10457
and Vibrio cholerae serogroup 037. Nuclear magnetic resonance (NMR)
spectroscopy was the primary technique employed.
S. marcescens O14:K12 contains both an O-antigen polysaccharide and a
capsular polysaccharide (K-antigen). The K-antigen was successfully
purified from the mixture of the two polysaccharides and was analysed.
The structure of the K-antigen is similar, but not identical, to other
K-antigens of S. marcescens In addition, the polysaccharide has two
acidic components, a pyruvic acid and a uronic acid, which is unusual.
The study is a step towards the identification of the serology of S.
marcescens.
New types of Hafnia alvei strains from Bangladesh have been reported to
cause severe diarrhoea. However, these strains also contain a virulence
factor that is characteristic for enteropathogenic Escherichia coli
(EPEC) making the serologic classification uncertain. The Hafnia strains
from Bangladesh may be pathogenic H. alvei, unusual biotypes of E. coli,
or represent a new species in the genus Escherichia. From the data on the
structure of the 0antigen, it may be classified as either of the two with
regard to carbohydrate structure. An unusual feature, however, is the
terminally linked N-acetyl neuraminic acid, which is a common constituent
in glycoproteins and glycolipids.
Vibrio cholerae serogroup 037 has genetic relationships with the cholera
causing V. cholerae serogroups 01 and 0139. This prompted us to
investigate the O-antigen structure from V. cholerae 037 Sudan strain.
The structure of the repeating unit was established, which differed
significantly from 01 and 0139.
The last part of this thesis describes an improved method for cleaving
off lipid A from the LPS. The procedure was shown to work on LPS from.S.
marcescens O14:K12, H. alvei strain 10457, and E. coli 0128. This method
involves ceric ammonium nitrate (CAN) in water and may be a useful
alternative if polysaccharides from LPS contain acid-labile constituents
that might be cleaved off during normal delipidation conditions.
